Expression of green fluorescent protein in human foreskin fibroblasts for use in 2D and 3D culture models

被引：22

作者：

Chao, Jie ^{[1
]}

Pena, Tiffany ^{[1
]}

Heimann, Dean G. ^{[1
]}

Hansen, Chris ^{[1
]}

Doyle, David A. ^{[1
]}

Yanala, Ujwal R. ^{[1
]}

Guenther, Timothy M. ^{[1
]}

Carlson, Mark A. ^{[1
,2
]}

机构：

[1] Univ Nebraska Med Ctr, Dept Surg, Omaha, NE USA

[2] VA Nebraska Western Iowa Hlth Care Syst, Omaha, NE USA

来源：

WOUND REPAIR AND REGENERATION | 2014年 / 22卷 / 01期

关键词：

COLLAGEN MATRIX CONTRACTION; VIABILITY SIGNALING PATHWAY; MESENCHYMAL STEM-CELLS; 3-DIMENSIONAL MATRICES; MIGRATION; SURVIVAL; RELEASE; KINASE; INTEGRATION; ACTIVATION;

D O I：

10.1111/wrr.12121

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The availability of fibroblasts that express green fluorescent protein (GFP) would be of interest for the monitoring of cell growth, migration, contraction, and other processes within the fibroblast-populated collagen matrix and other culture systems. A plasmid lentiviral vector-GFP (pLV-GFP) was utilized for gene delivery to produce primary human foreskin fibroblasts (HFFs) that stably express GFP. Cell morphology, cell migration, and collagen contraction were compared between nontransduced HFFs and transduced GFP-HFFs; no differences were observed. Immunocytochemical staining showed no differences in cell morphology between nontransduced and GFP-HFFs in both two-dimensional and three-dimensional culture systems. Furthermore, there was no significant difference in cellular population growth within the collagen matrix populated with nontransduced vs. GFP-HFFs. Within the limits of our assays, we conclude that transduction of GFP into HFFs did not alter the observed properties of HFFs compared with nontransduced fibroblasts. The GFP-HFFs may represent a new tool for the convenient monitoring of living primary fibroblast processes in two-dimensional or three-dimensional culture.

引用

页码：134 / 140

页数：7

共 28 条

[1]

Modulation of FAK, Akt, and p53 by stress release of the fibroblast-populated collagen matrix [J].