Effect of NaCl, glutathione and ascorbic acid on function of tonoplast vesicles isolated from barley leaves

被引:18
作者
Chen, Q [1 ]
Zhang, WH [1 ]
Liu, YL [1 ]
机构
[1] Nanjing Agr Univ, Dept Agron, Nanjing 210095, Peoples R China
关键词
AsA; GSH; Hordeum vulgare L; Tonoplast H+-ATPase; Tonoplast H+-PPase; salt stress;
D O I
10.1016/S0176-1617(99)80083-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The seedlings of two barley cultivars differing in salt sensitivity were treated with 300 mmol/L NaCl for 0, 2, 4, 6, and 8 days. The activities of H+-ATPase (EC 3.6.1.35) and H+-PPase (EC 3.6.1.1) associated with tonoplast vesicles isolated from leaves were assayed. H+-ATPase activity increased in the salt-tolerant cultivar during the first 4 days of NaCl stress, and decreased later. In the salt-sensitive cultivar, however, H+-ATPase activity decreased after NaCl treatment. Both GSH content and APX activity of barley seedling leaves in the two cultivars showed the same trends as H+-ATPase activity. There was a significantly positive correlation between H+-ATPase activity and APX activity and between H+-ATPase activity and GSH content, respectively Under salt stress, exogenous GSH or AsA stimulated the activities of H+-ATPase and H+-PPase in two cultivars, and diminished MDA content and electrolyte leakage in leaves of two cultivars.
引用
收藏
页码:685 / 690
页数:6
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