A combined photolithographic and molecular-assembly approach to produce functional micropatterns for applications in the biosciences

被引:156
作者
Falconnet, D [1 ]
Koenig, A [1 ]
Assi, T [1 ]
Textor, M [1 ]
机构
[1] ETH Honggerberg, Swiss Fed Inst Technol, Dept Mat, ETH Zurich,BioInterface Grp,Lab Surface Sci & Tec, CH-8093 Zurich, Switzerland
关键词
D O I
10.1002/adfm.200305182
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Chemical patterns have attracted substantial interest for applications in the field of biosensors, fundamental cell-surface interaction studies, tissue engineering, and biomaterials. A novel micropatterning technique is proposed here that combines a top-down approach based on photolithography and a bottom-up strategy through self-organization of multifunctional molecules. The development of the molecular-assembly patterning by lift-off (MAPL) has been driven by the need to economically produce patches incorporating a controlled surface density of bioligands while inhibiting non-specific adsorption. In the MAPL process, a photoresist pattern is transferred into the desired biochemical pattern by means of spontaneous adsorption of biologically relevant species and photoresist lift-off. The surface between the interactive patches is subsequently rendered non-fouling through immobilization of a polycationic poly(ethylene glycol) (PEG)-graft polymer. We demonstrate that surface density of biotin molecules inside adhesive islands can be tailored quantitatively and that cells grow selectively on cell-adhesive pepticle patterns. MAPL is considered to be a valuable addition to the toolbox of soft-lithography techniques for life-science applications combining simplicity (no clean-room equipment needed), cost-effectiveness, reproducibility on the scale of whole wafer surfaces, and flexibility in terms of pattern geometry, chemistry, and substrate choice.
引用
收藏
页码:749 / 756
页数:8
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