Determination of matrine and oxymatrine in Sophora subprostata by CE

被引:39
作者
Ku, YR
Chang, LY
Lin, JH
Ho, LK
机构
[1] Natl Labs Foods & Drugs, Dept Hlth, Taipei, Taiwan
[2] Natl Yang Ming Univ, Dept Pharmacol, Taipei, Taiwan
[3] Natl Yang Ming Univ, Inst Pharmacol, Taipei, Taiwan
关键词
matrine; oxymatrine; high-performance capillary electrophoresis (HPCE); pharmaceutical analysis;
D O I
10.1016/S0731-7085(02)00050-X
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Shan-dou-gen is the dried roots of Sophora subprostata (Leguminosae) and a commonly used Chinese herbal drug in Taiwan. It possesses antipyretic, anti-inflammatory, analgesic effects and is used to treat sore throat and acute pharyngolaryngeal infections. To evaluate the quality of S. subprostata, a simple, rapid and accurate high-performance capillary electrophoresis (HPCE) method was developed for the assay of two alkaloids: matrine and oxymatrine. The electrolyte was a buffer solution containing 75% 130 mM phosphate buffer (NaH2PO4/H3PO4, pH 3.5) and 25% acetonitrile. Applied voltage was 10 kV and temperature was 30 degreesC. 2-(4-Hydroxyphenyl)ethylammonium chloride was used as an internal standard and detector set at 200 nm. The contents of matrine and oxymatrine of S. subprostata in several different samples of crude drugs and commercial concentrated preparation have also been determined. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:1005 / 1010
页数:6
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