Validation of bioanalytical LC-MS/MS assays: Evaluation of matrix effects

被引：617

作者：

Van Eeckhaut, Ann ^{[1
]}

Lanckmans, Katrien ^{[1
]}

Sarre, Sophie ^{[1
]}

Smolders, Ilse ^{[1
]}

Michotte, Yvette ^{[1
]}

机构：

[1] Vrije Univ Brussel, Dept Pharmaceut Chem Drug Anal & Drug Informat, B-1090 Brussels, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 23期

关键词：

Matrix effects; Liquid chromatography; Tandem mass spectrometry; Bioanalysis; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; LABELED INTERNAL STANDARD; HUMAN PLASMA; ION SUPPRESSION; QUANTITATIVE-DETERMINATION; SIMULTANEOUS QUANTIFICATION; SAMPLE PREPARATION; HUMAN SERUM; DOG PLASMA;

D O I：

10.1016/j.jchromb.2009.01.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography Coupled to atmospheric pressure ionization tandem mass spectrometry is currently the method of choice for the quantitative determination of drugs in biological matrices. The advantages of this technique include high specificity, sensitivity and throughput. However, co-eluting matrix components, which are not observed in the chromatogram, can have a detrimental effect on the analysis, since they can cause ion Suppression or enhancement of the analyte. The evaluation of matrix effects on the quantitative analysis of drugs in biological fluids is an important and sometimes overlooked aspect of assay validation. In this review, the influence of matrix effects on bioanalytical LC-MS/MS methods is discussed and illustrated with some examples. In addition, possible solutions to reduce or eliminate matrix effects are highlighted. A literature overview of validated LC-MS/MS methods published from January till June 2008 is also included. Although matrix effects are investigated in most papers, there is no consensus on how matrix effects should be evaluated during method validation. In addition, the definition of specificity should be changed for LC-MS/MS based methods. (C) 2009 Elsevier B.V. All rights reserved

引用

页码：2198 / 2207

页数：10

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