Defined human system that supports bidirectional mismatch-provoked excision

被引：190

作者：

Dzantiev, L

Constantin, N

Genschel, J

Iyer, RR

Burgers, PM

Modrich, P

机构：

[1] Duke Univ, Ctr Med, Howard Hughes Med Inst, Durham, NC 27710 USA

[2] Duke Univ, Ctr Med, Dept Biochem, Durham, NC 27710 USA

[3] Washington Univ, Sch Med, Dept Biochem, St Louis, MO 63110 USA

来源：

MOLECULAR CELL | 2004年 / 15卷 / 01期

关键词：

D O I：

10.1016/j.molcel.2004.06.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.

引用

收藏

页码：31 / 41

页数：11

相关论文

共 53 条

[1] The coordinated functions of the E-coli MutS and MutL proteins in mismatch repair [J].

Acharya, S ;

Foster, PL ;

Brooks, P ;

Fishel, R .

MOLECULAR CELL, 2003, 12 (01) :233-246

[2] DNA recognition properties of the N-terminal DNA binding domain within the large subunit of Replication Factor C [J].

Allen, BL ;

Uhlmann, F ;

Gaur, LK ;

Mulder, BA ;

Posey, KL ;

Jones, LB ;

Hardin, SH .

NUCLEIC ACIDS RESEARCH, 1998, 26 (17) :3877-3882

[3] MutS mediates heteroduplex loop formation by a translocation mechanism [J].

Allen, DJ ;

Makhov, A ;

Grilley, M ;

Taylor, J ;

Thresher, R ;

Modrich, P ;

Griffith, JD .

EMBO JOURNAL, 1997, 16 (14) :4467-4476

[4] exo1-Dependent mutator mutations:: Model system for studying functional interactions in mismatch repair [J].

Amin, NS ;

Nguyen, MN ;

Oh, S ;

Kolodner, RD .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (15) :5142-5155

[5]

AYYAGARI R, 1995, MOL CELL BIOL, V15, P4420

[6] Functional interactions and signaling properties of mammalian DNA mismatch repair proteins [J].

Bellacosa, A .

CELL DEATH AND DIFFERENTIATION, 2001, 8 (11) :1076-1092

[7] DNA chain length dependence of formation and dynamics of hMutSα.hMutLα.heteroduplex complexes [J].

Blackwell, LJ ;

Wang, ST ;

Modrich, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (35) :33233-33240

[8] Mammalian DNA mismatch repair [J].

Buermeyer, AB ;

Deschênes, SM ;

Baker, SM ;

Liskay, RM .

ANNUAL REVIEW OF GENETICS, 1999, 33 :533-564

[9] In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair [J].

Burdett, V ;

Baitinger, C ;

Viswanathan, M ;

Lovett, ST ;

Modrich, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (12) :6765-6770

[10] Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex [J].

Dao, V ;

Modrich, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) :9202-9207

← 1 2 3 4 5 6 →