Defined human system that supports bidirectional mismatch-provoked excision

被引：190

作者：

Dzantiev, L

Constantin, N

Genschel, J

Iyer, RR

Burgers, PM

Modrich, P

机构：

[1] Duke Univ, Ctr Med, Howard Hughes Med Inst, Durham, NC 27710 USA

[2] Duke Univ, Ctr Med, Dept Biochem, Durham, NC 27710 USA

[3] Washington Univ, Sch Med, Dept Biochem, St Louis, MO 63110 USA

来源：

MOLECULAR CELL | 2004年 / 15卷 / 01期

关键词：

D O I：

10.1016/j.molcel.2004.06.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mismatch-provoked excision directed by a strand break located 3' or 5' to the mispair has been reconstituted using purified human proteins. While MutSalpha, EXOI, and RPA are sufficient to support hydrolysis directed by a 5' strand break, 3' directed excision also requires MutLalpha, PCNA, and RFC. EXOI interacts with PCNA. RFC and PCNA suppress EXOI-mediated 5' to 3' hydrolysis when the nick that directs excision is located 3' to the mispair and activate 3' to 5' excision, which is dependent on loaded PCNA and apparently mediated by a cryptic EXOI 3' to 5' hydrolytic function. By contrast, RFC and PCNA have only a limited effect on 5' to 3' excision directed by a 5' strand break.

引用

收藏

页码：31 / 41

页数：11

相关论文

共 53 条

[11] HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and MSh6 mismatch-repair protein functions [J].

de Wind, N ;

Dekker, M ;

Claij, N ;

Jansen, L ;

van Klink, Y ;

Radman, M ;

Riggins, G ;

van der Valk, M ;

van 't Wout, K ;

Riele, HT .

NATURE GENETICS, 1999, 23 (03) :359-362

[12]

Edelmann W, 2000, CANCER RES, V60, P803

[13]

FANG WH, 1993, J BIOL CHEM, V268, P11838

[14] IDENTIFICATION OF REPLICATION FACTOR-C FROM SACCHAROMYCES-CEREVISIAE - A COMPONENT OF THE LEADING-STRAND DNA-REPLICATION COMPLEX [J].

FIEN, K ;

STILLMAN, B .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (01) :155-163

[15] A conserved domain of the large subunit of replication factor C binds PCNA and acts like a dominant negative inhibitor of DNA replication in mammalian cells [J].

Fotedar, R ;

Mossi, R ;

Fitzgerald, P ;

Rousselle, T ;

Maga, G ;

Brickner, H ;

Messier, H ;

Kasibhatla, S ;

Hubscher, U ;

Fotedar, A .

EMBO JOURNAL, 1996, 15 (16) :4423-4433

[16] ATP hydrolysis-dependent formation of a dynamic ternary nucleoprotein complex with MutS and MutL [J].

Galio, L ;

Bouquet, C ;

Brooks, P .

NUCLEIC ACIDS RESEARCH, 1999, 27 (11) :2325-2331

[17] Isolation of MutSβ from human cells and comparison of the mismatch repair specificities of MutSβ and MutSα [J].

Genschel, J ;

Littman, SJ ;

Drummond, JT ;

Modrich, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (31) :19895-19901

[18] Mechanism of 5′-directed excision in human mismatch repair [J].

Genschel, J ;

Modrich, P .

MOLECULAR CELL, 2003, 12 (05) :1077-1086

[19] Human exonuclease I is required for 5′ and 3′ mismatch repair. [J].

Genschel, J ;

Bazemore, LR ;

Modrich, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (15) :13302-13311

[20] Overproduction and affinity purification of Saccharomyces cerevisiae replication factor C [J].

Gerik, KJ ;

Gary, SL ;

Burgers, PMJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (02) :1256-1262

← 1 2 3 4 5 6 →