Arabidopsis DOF Transcription Factors Act Redundantly to Reduce CONSTANS Expression and Are Essential for a Photoperiodic Flowering Response

被引:532
作者
Fornara, Fabio [1 ]
Panigrahi, Kishore C. S. [1 ]
Gissot, Lionel [1 ]
Sauerbrunn, Nicolas [1 ]
Ruehl, Mark [1 ]
Jarillo, Jose A. [2 ]
Coupland, George [1 ]
机构
[1] Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany
[2] Ctr Biotecnol & Genom Plantas INIA UAU, Inst Nacl Invest & Tecnol Agr & Alimentaria, Dept Biotecnol, Madrid 28040, Spain
关键词
CLOCK-CONTROLLED GENE; CIRCADIAN CLOCK; FLORAL INDUCTION; FT PROTEIN; LOCUS-T; TARGETED DEGRADATION; MOLECULAR-BASIS; BLUE-LIGHT; GIGANTEA; TIME;
D O I
10.1016/j.devcel.2009.06.015
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Flowering of Arabidopsis is induced by long summer days (LDs). The transcriptional regulator CONSTANS (CO) promotes flowering, and its transcription is increased under LDs. We systematically misexpressed transcription factors in companion cells and identified several DOF proteins that delay flowering by repressing CO transcription. Combining mutations in four of these, including CYCLING DOF FACTOR 2 (CDF2), caused photoperiod-insensitive early flowering by increasing CO mRNA levels. CO transcription is promoted to differing extents by GIGANTEA (GI) and the F-box protein FKF1. We show that GI stabilizes FKF1, thereby reducing CDF2 abundance and allowing transcription of CO. Despite the crucial function of GI in wild-type plants, introducing mutations in the four DOF-encoding genes into gi mutants restored the diurnal rhythm and light inducibility of CO. Thus, antagonism between GI and DOF transcription factors contributes to photoperiodic flowering by modulating an underlying diurnal rhythm in CO transcript levels.
引用
收藏
页码:75 / 86
页数:12
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