Phosphopeptide enrichment by IEF

被引：17

作者：

Maccarrone, Giuseppina ^{[1
]}

Kolb, Nikolaus ^{[1
]}

Teplytska, Larysa ^{[1
]}

Birg, Isabel ^{[1
]}

Zollinger, Richard ^{[1
]}

Holsboer, Florian ^{[1
]}

Turck, Christoph W. ^{[1
]}

机构：

[1] Max Planck Inst Psychiat, D-80804 Munich, Germany

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 22期

关键词：

peptide IEF; phosphopeptide enrichment; protein phosphorylation;

D O I：

10.1002/elps.200600145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In our efforts to improve the identification of phosphopeptides by MS we have used peptide IEF on IPG strips. Phosphopeptides derived from trypsin digests of single proteins as well as complex cellular protein mixtures can be enriched by IEF and recovered in excellent yields at the acidic end of an IPG strip. IPG peptide fractionation in combination with MS/MS analysis has allowed us to identify phosphopeptides from tryptic digests of a cellular protein extract.

引用

页码：4585 / 4595

页数：11

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