Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting

被引：57

作者：

Duncombe, Todd A. ^{[1
,2
]}

Kang, Chi-Chih ^{[1
]}

Maity, Santanu ^{[1
]}

Ward, Toby M. ^{[3
]}

Pegram, Mark D. ^{[3
]}

Murthy, Niren ^{[1
,2
]}

Herr, Amy E. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, UC Berkeley UCSF Grad Program Bioengn, Berkeley, CA 94720 USA

[3] Stanford Univ, Dept Med, Div Oncol, Stanford, CA 94305 USA

来源：

ADVANCED MATERIALS | 2016年 / 28卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

BREAST-CANCER; GEL-ELECTROPHORESIS; FLOW-CYTOMETRY; HETEROGENEITY; AMPLIFICATION; PROTEINS; IMMUNOASSAY; SEPARATION; SYSTEM; PH;

D O I：

10.1002/adma.201503939

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：327 / 334

页数：8

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