Diversity and expression of nitrogenase genes (nifH) from ectomycorrhizas of Corsican pine (Pinus nigra)

被引：30

作者：

Izumi, Hironari ^{[1
]}

Anderson, Ian C.

Alexander, Ian J.

Killham, Ken

Moore, Edward R. B.

机构：

[1] Macaulay Land Use Res Inst, Aberdeen AB15 8QH, Scotland

[2] Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 3UU, Scotland

[3] Univ Gothenburg, CCUG, Dept Clin Bacteriol, SE-41346 Gothenburg, Sweden

来源：

ENVIRONMENTAL MICROBIOLOGY | 2006年 / 8卷 / 12期

关键词：

D O I：

10.1111/j.1462-2920.2006.01104.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The diversity of bacterial nitrogenase genes (nifH) and their mRNA transcription in ectomycorrhizas of Corsican pine (Pinus nigra) were examined. DNA and RNA were extracted from surface-sterilized and non-sterilized Corsican pine roots colonized by the ectomycorrhizal (ECM) fungi, Suillus variegatus and Tomentellopsis submollis. DNA-derived nifH polymerase chain reaction (PCR) products were obtained from all samples, but only a few reverse transcription PCRs for nifH mRNA were successful, suggesting that nitrogenase genes were not always transcribed. Several different nifH sequences were detected and the bacteria actively transcribing nifH were different from those whose genes were detected through DNA-based PCR. Putative nitrogenase amino acid sequences revealed that more than half of the nifH products were derived from methylotrophic bacteria, such as Methylocella spp. The next most frequent sequence types were similar to those from Burkholderia.

引用

页码：2224 / 2230

页数：7

共 44 条

[11] Methylocapsa acidiphila gen. nov., sp nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from Sphagnum bog [J].

Dedysh, SN ;

Khmelenina, VN ;

Suzina, NE ;

Trotsenko, YA ;

Semrau, JD ;

Liesack, W ;

Tiedje, JM .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2002, 52 :251-261

[12] Quantifying nitrogen-fixation in feather moss carpets of boreal forests [J].

DeLuca, TH ;

Zackrisson, O ;

Nilsson, MC ;

Sellstedt, A .

NATURE, 2002, 419 (6910) :917-920

[13] Endophytic expression of nif genes of Azoarcus sp strain BH72 in rice roots [J].

Egener, T ;

Hurek, T ;

Reinhold-Hurek, B .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (09) :813-819

[14] Endophytic colonization and in planta nitrogen fixation by a Herbaspirillum sp isolated from wild rice species [J].

Elbeltagy, A ;

Nishioka, K ;

Sato, T ;

Suzuki, H ;

Ye, B ;

Hamada, T ;

Isawa, T ;

Mitsui, H ;

Minamisawa, K .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (11) :5285-5293

[15] Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution [J].

Estrada-De los Santos, P ;

Bustillos-Cristales, R ;

Caballero-Mellado, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2790-2798

[16] Mycorrhiza helper bacteria: a promising model for the genomic analysis of fungal-bacterial interactions [J].

Frey-Klett, P ;

Garbaye, J .

NEW PHYTOLOGIST, 2005, 168 (01) :4-8

[17] ITS PRIMERS WITH ENHANCED SPECIFICITY FOR BASIDIOMYCETES - APPLICATION TO THE IDENTIFICATION OF MYCORRHIZAE AND RUSTS [J].

GARDES, M ;

BRUNS, TD .

MOLECULAR ECOLOGY, 1993, 2 (02) :113-118

[18] ACETOBACTER-DIAZOTROPHICUS SP-NOV, A NITROGEN-FIXING ACETIC-ACID BACTERIUM ASSOCIATED WITH SUGARCANE [J].

GILLIS, M ;

KERSTERS, K ;

HOSTE, B ;

JANSSENS, D ;

KROPPENSTEDT, RM ;

STEPHAN, MP ;

TEIXEIRA, KRS ;

DOBEREINER, J ;

DELEY, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :361-364

[19] Contamination of diverse nifH and nifH-like DNA into commercial PCR primers [J].

Goto, M ;

Ando, S ;

Hachisuka, Y ;

Yoneyama, T .

FEMS MICROBIOLOGY LETTERS, 2005, 246 (01) :33-38

[20] Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens [J].

Gyaneshwar, P ;

James, EK ;

Mathan, N ;

Reddy, PM ;

Reinhold-Hurek, B ;

Ladha, JK .

JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2634-2645

← 1 2 3 4 5 →