Rapid determination of sugars, nonvolatile acids, and ascorbic acid in strawberry and other fruits

被引：158

作者：

Perez, AG ^{[1
]}

Olias, R ^{[1
]}

Espada, J ^{[1
]}

Olias, JM ^{[1
]}

Sanz, C ^{[1
]}

机构：

[1] CSIC,INST GRASA,DEPT FISIOL & TECNOL PROD VEGETALES,SEVILLE 41012,SPAIN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 09期

关键词：

simultaneous analysis; high-performance liquid chromatography; sugars; organic acids; vitamin C; strawberry; fruits;

D O I：

10.1021/jf9701704

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An analytical procedure to determine major sugars and organic acids, including vitamin C, in fruits was developed using a C-18 Sep-Pak cleanup process and an ion exclusion HPLC column. Dual UV monitoring and refractive index were performed for detection. To attain optimal separation and quantitation, 0.0085N H2SO4 was used as the mobile phase and the column temperature was maintained at 23 degrees C. This procedure was compared to others for the individual quantitation of sugars, organic acids, and vitamin C. Recovery and reproducibility of this analytical procedure were quite acceptable for strawberry and four other common fruits, allowing the analysis of all the components using a single-injection HPLC analysis in <22 min.

引用

页码：3545 / 3549

页数：5

共 34 条

[11] SIMULTANEOUS HPLC DETERMINATION OF ORGANIC-ACIDS, SUGARS AND ALCOHOLS [J].

DOYON, G ;

GAUDREAU, G ;

STGELAIS, D ;

BEAULIEU, Y ;

RANDALL, CJ .

CANADIAN INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY JOURNAL-JOURNAL DE L INSTITUT CANADIEN DE SCIENCE ET TECHNOLOGIE ALIMENTAIRES, 1991, 24 (1-2) :87-94

[12]

FORNEY CF, 1986, J AM SOC HORTIC SCI, V111, P241

[13] DETECTION OF ADDED L-ASCORBIC-ACID IN FRUIT JUICES BY ISOTOPE RATIO MASS-SPECTROMETRY [J].

GENSLER, M ;

ROSSMANN, A ;

SCHMIDT, HL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (10) :2662-2666

[14] DETERMINATION OF ASCORBIC AND DEHYDROASCORBIC ACID IN POTATOES (SOLANUM-TUBEROSUM) AND STRAWBERRIES USING ION-EXCLUSION CHROMATOGRAPHY [J].

GRAHAM, WD ;

ANNETTE, D .

JOURNAL OF CHROMATOGRAPHY, 1992, 594 (1-2) :187-194

[15]

Horwitz W., 1980, OFF METH AN ASS OFF, P746

[16]

HUDSON DE, 1985, HORTSCIENCE, V20, P71

[17] SPECIFIC DETERMINATION OF ASCORBIC-ACID WITH CHEMICAL DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KISHIDA, E ;

NISHIMOTO, Y ;

KOJO, S .

ANALYTICAL CHEMISTRY, 1992, 64 (13) :1505-1507

[18]

KISSINGER PT, 1987, FOOD TECHNOL-CHICAGO, V41, P108

[19] SIMULTANEOUS DETERMINATION OF ASCORBIC-ACID, CAFFEINE AND PARACETAMOL IN DRUG FORMULATIONS BY DIFFERENTIAL-PULSE VOLTAMMETRY USING A GLASSY-CARBON ELECTRODE [J].

LAU, OW ;

LUK, SF ;

CHEUNG, YM .

ANALYST, 1989, 114 (09) :1047-1051

[20] RAPID METHOD FOR THE SIMULTANEOUS GC QUANTITATION OF ACIDS AND SUGARS IN FRUITS AND VEGETABLES [J].

MOLNARPERL, I ;

MORVAI, M .

FOOD ADDITIVES AND CONTAMINANTS, 1992, 9 (05) :505-514

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