Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level

被引：87

作者：

Keller, Martin ^{[1
]}

Hettich, Robert ^{[2
]}

机构：

[1] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

[2] Oak Ridge Natl Lab, Div Chem Sci, Oak Ridge, TN 37831 USA

来源：

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS | 2009年 / 73卷 / 01期

关键词：

MASS-SPECTROMETRY; GEL-ELECTROPHORESIS; COMMUNITY PROTEOMICS; DIRECT EXTRACTION; LC-MS; SOIL; REACTOR; METAPROTEOMICS; IDENTIFICATION; MICROCHIP;

D O I：

10.1128/MMBR.00028-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The increase in sequencing capacity led to a new wave of metagenomic projects, enabling and setting the prerequisite for the application of environmental proteomics technologies. This review describes the current status of environmental proteomics. It describes sample preparation as well as the two major technologies applied within this field: two-dimensional electrophoresis-based environmental proteomics and liquid chromatography-mass spectrometry-based environmental proteomics. It also highlights current publications and describes major scientific findings. The review closes with a discussion of critical improvements in the area of integrating experimental mass spectrometry technologies with bioinformatics as well as improved sample handling.

引用

页码：62 / +

页数：10

共 68 条

[11] Metagenomic analysis of the human distal gut microbiome [J].

Gill, Steven R. ;

Pop, Mihai ;

DeBoy, Robert T. ;

Eckburg, Paul B. ;

Turnbaugh, Peter J. ;

Samuel, Buck S. ;

Gordon, Jeffrey I. ;

Relman, David A. ;

Fraser-Liggett, Claire M. ;

Nelson, Karen E. .

SCIENCE, 2006, 312 (5778) :1355-1359

[12] Biology's next revolution [J].

Goldenfeld, Nigel ;

Woese, Carl .

NATURE, 2007, 445 (7126) :369-369

[13] Molecular biologist's guide to proteomics [J].

Graves, PR ;

Haystead, TAJ .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2002, 66 (01) :39-+

[14] Capturing the uncultivated majority [J].

Green, Brian D. ;

Keller, Martin .

CURRENT OPINION IN BIOTECHNOLOGY, 2006, 17 (03) :236-240

[15] Whole proteome analysis of post-translational modifications: Applications of mass-spectrometry for proteogenomic annotation [J].

Gupta, Nitin ;

Tanner, Stephen ;

Jaitly, Navdeep ;

Adkins, Joshua N. ;

Lipton, Mary ;

Edwards, Robert ;

Romine, Margaret ;

Osterman, Andrei ;

Bafna, Vineet ;

Smith, Richard D. ;

Pevzner, Pavel A. .

GENOME RESEARCH, 2007, 17 (09) :1362-1377

[16]

Hanash SM, 2000, ELECTROPHORESIS, V21, P1202, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1202::AID-ELPS1202>3.0.CO

[17]

2-I

[18] Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products [J].

Handelsman, J ;

Rondon, MR ;

Brady, SF ;

Clardy, J ;

Goodman, RM .

CHEMISTRY & BIOLOGY, 1998, 5 (10) :R245-R249

[19] Chip-LC-MS for label-free profiling of human serum [J].

Horvatovich, Peter ;

Govorukhina, Natalia . ;

Reijmers, Theo H. ;

van der Zee, Ate G. J. ;

Suits, Frank ;

Bischoff, Rainer .

ELECTROPHORESIS, 2007, 28 (23) :4493-4505

[20] Ammonia-oxidizing bacteria respond to multifactorial global change [J].

Horz, HP ;

Barbrook, A ;

Field, CB ;

Bohannan, BJM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (42) :15136-15141

← 1 2 3 4 5 6 7 →