Adipose-derived mesenchymal stem cells release microvesicles with procoagulant activity

被引：29

作者：

Fiedler, Tomas ^{[1
]}

Rabe, Magdalena ^{[2
]}

Mundkowski, Ralf G. ^{[3
]}

Oehmcke-Hecht, Sonja ^{[1
]}

Peters, Kirsten ^{[2
]}

机构：

[1] Rostock Univ Med Ctr, Inst Med Microbiol Virol & Hyg, Schillingallee 70, D-18057 Rostock, Germany

[2] Rostock Univ Med Ctr, Dept Cell Biol, Schillingallee 69, D-18057 Rostock, Germany

[3] Rostock Univ Med Ctr, Ctr Pharmacol & Toxicol, Inst Clin Pharmacol, Rostock, Germany

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2018年 / 100卷

关键词：

Mesenchymal stem cells; Extracellular vesicles; Coagulation; LPS; TNF; Microvesicles; BLOOD MONONUCLEAR-CELLS; EXTRACELLULAR VESICLES; STROMAL CELLS; CANCER-PATIENTS; IN-VITRO; TISSUE; MICROPARTICLES; DIFFERENTIATION; THERAPY; DISEASE;

D O I：

10.1016/j.biocel.2018.05.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Extracellular vesicles are produced by a number of different cell types, among them mesenchymal stromal/stem cells (MSC) of different sources. It has been shown that extracellular vesicles of MSC exert similar therapeutic effects as the cells themselves. Here, we isolated and characterized extracellular vesicles produced by adipose-derived MSC (adMSC) in vitro upon stimulation with the proinflammatory substances lipopolysaccharide (LPS) and tumor necrosis factor (TNF). We found that the number of vesicles produced by adMSC does not change upon stimulation of the cells with LPS and TNF. Furthermore, adMSC-derived extracellular vesicles exert pro-coagulant activity independent of previous stimulation with LPS or TNF. We found evidence that the vesicles induce coagulation via both the intrinsic and the extrinsic pathway of coagulation.

引用

收藏

页码：49 / 53

页数：5

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