Physcomitrella patens has lipoxygenases for both eicosanoid and octadecanoid pathways

被引：39

作者：

Anterola, Aldwin ^{[1
]}

Goebel, Cornelia ^{[2
]}

Hornung, Ellen ^{[2
]}

Sellhorn, George ^{[3
]}

Feussner, Ivo ^{[2
]}

Grimes, Howard ^{[3
]}

机构：

[1] So Illinois Univ, Carbondale, IL 62901 USA

[2] Univ Gottingen, D-37077 Gottingen, Germany

[3] Washington State Univ, Pullman, WA 99164 USA

来源：

PHYTOCHEMISTRY | 2009年 / 70卷 / 01期

关键词：

Physcomitrella patens; Funariaceae; Lipoxygenase; Dioxygenase; Lipid peroxidation; Oxylipin formation; pH dependent positional specificity; Substrate orientation; Eicosanoid; ARACHIDONIC-ACID; RED ALGA; LINOLENIC ACID; BIOSYNTHESIS; OXYLIPINS; METABOLISM; PLANTS; LAND;

D O I：

10.1016/j.phytochem.2008.11.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mosses have substantial amounts of long chain C20 polyunsaturated fatty acids, such as arachidonic and eicosapentaenoic acid, in addition to the shorter chain C18 alpha-linolenic and linoleic acids, which are typical substrates of lipoxygenases in flowering plants. To identify the fatty acid substrates used by moss lipoxygenases, eight lipoxygenase genes from Physcomitrella patens were heterologously expressed in Escherichia coli, and then analyzed for lipoxygenase activity using linoleic, alpha-linolenic and arachidonic acids as substrates. Among the eight moss lipoxygenases, only seven were found to be enzymatically active in vitro, two of which selectively used arachidonic acid as the substrate, while the other five preferred alpha-linolenic acid. Based on enzyme assays using a Clark-type oxygen electrode, all of the active lipoxygenases had an optimum pH at 7.0, except for one with highest activity at pH 5.0. HPLC analyses indicated that the two arachidonic acid lipoxygenases form (12S)-hydroperoxy eicosatetraenoic acid as the main product, while the other five lipoxygenases produce mainly (13S)-hydroperoxy octadecatrienoic acid from alpha-linolenic acid. These results suggest that mosses may have both C20 and C18 based oxylipin pathways. Published by Elsevier Ltd.

引用

页码：40 / 52

页数：13

共 56 条

[11] The innate immunity of a marine red alga involves oxylipins from both the eicosanoid and octadecanoid pathways [J].