Transient over-expression of barley BAX Inhibitor-1 weakens oxidative defence and MLA12-mediated resistance to Blumeria graminis f.sp hordei

被引:25
作者
Eichmann, Ruth [1 ]
Dechert, Cornelia [1 ]
Kogel, Karl-Heinz [1 ]
Hueckelhoven, Ralph [1 ]
机构
[1] Univ Giessen, Res Ctr BioSyst, Inst Phytopathol & Appl Zool, D-35392 Giessen, Germany
关键词
D O I
10.1111/j.1364-3703.2006.00359.X
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
BAX Inhibitor-1 (BI-1) is a conserved cell death suppressor protein. In barley, BI-1 (HvBI-1) expression is induced upon powdery mildew infection and when over-expressed in epidermal cells of barley, HvBI-1 induces susceptibility to the biotrophic fungal pathogen Blumeria graminis. We co-expressed mammalian pro-apoptotic BAX together with HvBI-1, and the mammalian BAX antagonist BCL-X-L in barley epidermal cells. BAX expression led to cessation of cytoplasmic streaming and collapse of the cytoplasm while co-expression of HvBI-1 and BCL-X-L partially or completely, respectively, rescued cells from BAX lethality. When B. graminis was attacking epidermal cells, a green fluorescent protein fusion of HvBI-1 accumulated at the site of attempted penetration and was also present around haustoria. Over-expression of HvBI-1 in epidermal cells weakened a cell-wall-associated local hydrogen peroxide burst in a resistant mlo-mutant genotype and supported haustoria accommodation in race-specifically resistant MLA12-barley. HvBI-1 is a cell death regulator protein of barley with the potential to suppress host defence reactions.
引用
收藏
页码:543 / 552
页数:10
相关论文
共 54 条
[41]   Functional analysis of barley RAC/ROP G-protein family members in susceptibility to the powdery mildew fungus [J].
Schultheiss, H ;
Dechert, C ;
Kogel, KH ;
Hückelhoven, R .
PLANT JOURNAL, 2003, 36 (05) :589-601
[42]   Establishment of biotrophy by parasitic fungi and reprogramming of host cells for disease resistance [J].
Schulze-Lefert, P ;
Panstruga, R .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2003, 41 :641-667
[43]   A transient assay system for the functional assessment of defense-related genes in wheat [J].
Schweizer, P ;
Pokorny, J ;
Abderhalden, O ;
Dudler, R .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1999, 12 (08) :647-654
[44]   BAX and BAK regulation of endoplasmic reticulum Ca2+:: A control point for apoptosis [J].
Scorrano, L ;
Oakes, SA ;
Opferman, JT ;
Cheng, EH ;
Sorcinelli, MD ;
Pozzan, T ;
Korsmeyer, SJ .
SCIENCE, 2003, 300 (5616) :135-139
[45]   Recognition specificity and RAR1/SGT1 dependence in barley Mla disease resistance genes to the powdery mildew fungus [J].
Shen, QH ;
Zhou, FS ;
Bieri, S ;
Haizel, T ;
Shirasu, K ;
Schulze-Lefert, P .
PLANT CELL, 2003, 15 (03) :732-744
[46]   Cell-autonomous complementation of mlo resistance using a biolistic transient expression system [J].
Shirasu, K ;
Nielsen, K ;
Piffanelli, P ;
Oliver, R ;
Schulze-Lefert, P .
PLANT JOURNAL, 1999, 17 (03) :293-299
[47]   Ultrastructural characterisation of the host-pathogen interface in white blister-infected Arabidopsis leaves [J].
Soylu, S .
MYCOPATHOLOGIA, 2004, 158 (04) :457-464
[48]   Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration [J].
Stein, M ;
Dittgen, J ;
Sánchez-Rodríguez, C ;
Hou, BH ;
Molina, A ;
Schulze-Lefert, P ;
Lipka, V ;
Somerville, S .
PLANT CELL, 2006, 18 (03) :731-746
[49]   Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction [J].
ThordalChristensen, H ;
Zhang, ZG ;
Wei, YD ;
Collinge, DB .
PLANT JOURNAL, 1997, 11 (06) :1187-1194
[50]   Arabidopsis Bax inhibitor-1 functions as an attenuator of biotic and abiotic types of cell death [J].
Watanabe, N ;
Lam, E .
PLANT JOURNAL, 2006, 45 (06) :884-894