An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria

被引：71

作者：

Mangan, JA ^{[1
]}

Sole, KM ^{[1
]}

Mitchison, DA ^{[1
]}

Butcher, PD ^{[1
]}

机构：

[1] ST GEORGE HOSP,SCH MED,DEPT MED MICROBIOL,LONDON SW17 0RE,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/25.3.675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 mu g RNA/10(9) Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.

引用

页码：675 / 676

页数：2

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