In vivo angiotensin I-converting enzyme inhibition by long-term intake of antihypertensive lactoferrin hydrolysate in spontaneously hypertensive rats

被引:39
作者
Fernandez-Musoles, Ricardo [1 ]
Manzanares, Paloma [1 ]
Burguete, Maria C. [3 ,4 ]
Alborch, Enrique [2 ,3 ,4 ]
Salom, Juan B. [2 ,3 ,4 ]
机构
[1] CSIC, Inst Agroquim & Tecnol Alimentos, Dept Biotecnol Alimentos, Valencia 46980, Spain
[2] Hosp Univ La Fe, Ctr Invest, Valencia 46009, Spain
[3] Univ Valencia, Dept Fisiol, Valencia 46010, Spain
[4] Univ Valencia, Unidad Mixta Invest Cerebrovasc, IIS Fdn Invest Hosp La Fe, Valencia 46010, Spain
关键词
Bovine lactoferrin; Milk protein hydrolysate; Antihypertensive peptides; Renin-angiotensin system; In vivo ACE inhibition; Spontaneously hypertensive rat; PROTEIN HYDROLYSATE; BLOOD-PRESSURE; PEPTIDES; IDENTIFICATION; PREVENTS;
D O I
10.1016/j.foodres.2013.07.063
中图分类号
TS2 [食品工业];
学科分类号
100403 [营养与食品卫生学];
摘要
We evaluated the effect of the long-term intake of a bovine lactoferrin hydrolysate enriched in low molecular weight peptides (LFH < 3 kDa) on the progression of hypertension in spontaneously hypertensive rats and its in vivo mechanism of action. Male spontaneously hypertensive rats received tap water (negative control), captopril (positive control, 76 mg/kg/day), LFH < 3 kDa (285 mg/kg/day), or LFH < 3 kDa (782 mg/kg/day). Twice a week, systolic blood pressure, body weight and liquid intake were measured. Blood components of the renin-angiotensin system were determined. The systolic blood pressure of spontaneously hypertensive rats receiving water increased along the 24-day follow-up period, while systolic blood pressure around or slightly below the baseline was maintained in both LFH < 3 kDa treated groups. The antihypertensive effects appeared earlier in the high-dose than in the low-dose group. There were no differences in body weight gain between the two groups but liquid intake increased in rats treated with high-dose LFH < 3 kDa. There were reductions of circulating angiotensin I-converting enzyme activity, angiotensin 11 and aldosterone levels, as well as a compensatory increase of renin activity after LFH < 3 kDa intake. Our results support angiotensin I-converting enzyme inhibition as an in vivo mechanism for the antihypertensive effect of long-term oral administration of LFH < 3 kDa to spontaneously hypertensive rats and its potential application as a nutraceutical approach for hypertension treatment. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:627 / 632
页数:6
相关论文
共 35 条
[1]
BRUNNER HR, 1993, J HYPERTENS, V11, pS53
[2]
Newly recognized components of the renin-angiotensin system: Potential roles in cardiovascular and renal regulation [J].
Carey, RM ;
Siragy, HM .
ENDOCRINE REVIEWS, 2003, 24 (03) :261-271
[3]
Lactoferricin-related peptides with inhibitory effects on ACE-dependent vasoconstriction [J].
Centeno, Jose M. ;
Burguete, Maria C. ;
Castello-Ruiz, Maria ;
Enrique, Maria ;
Valles, Salvador ;
Salom, Juan B. ;
Torregrosa, German ;
Marcos, Jose F. ;
Alborch, Enrique ;
Manzanares, Paloma .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (15) :5323-5329
[4]
The angiotensin converting enzyme (ACE) [J].
Coates, D .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2003, 35 (06) :769-773
[6]
The antihypertensive effect of peptides: A novel alternative to drugs? [J].
Fang Hong ;
Luo Ming ;
Sheng Yi ;
Li Zhanxia ;
Wu Yongquan ;
Liu Chi .
PEPTIDES, 2008, 29 (06) :1062-1071
[7]
Antihypertensive effects of lactoferrin hydrolyzates: Inhibition of angiotensin and endothelin-converting enzymes [J].
Fernandez-Musoles, Ricardo ;
Salom, Juan B. ;
Martinez-Maqueda, Daniel ;
Javier Lopez-Diez, Jose ;
Recio, Isidra ;
Manzanares, Paloma .
FOOD CHEMISTRY, 2013, 139 (1-4) :994-1000
[8]
Angiotensin, thirst, and sodium appetite [J].
Fitzsimons, JT .
PHYSIOLOGICAL REVIEWS, 1998, 78 (03) :583-686
[9]
Pathophysiologic therapeutic targets in hypertension: a cardiological point of view [J].
Fragasso, Gabriele ;
Maranta, Francesco ;
Montanaro, Claudia ;
Salerno, Anna ;
Torlasco, Camilla ;
Margonato, Alberto .
EXPERT OPINION ON THERAPEUTIC TARGETS, 2012, 16 (02) :179-193
[10]
Lactoferrin: structure, function and applications [J].
Gonzalez-Chavez, Susana A. ;
Arevalo-Gallegos, Sigifredo ;
Rascon-Cruz, Quintin .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2009, 33 (04)