Phagocytosed Bordetella pertussis fails to survive in human neutrophils

被引:37
作者
Lenz, DH [1 ]
Weingart, CL [1 ]
Weiss, AA [1 ]
机构
[1] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45267 USA
关键词
D O I
10.1128/IAI.68.2.956-959.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Previous studies have reported that phagocytosed Bordetella pertussis sun ives in human neutrophils. This issue has been reexamined. Opsonized or unopsonized bacteria expressing green fluorescent protein (GFP) were incubated with adherent human neutrophils. Phagocytosis was quantified by fluorescence microscopy, and the viability of phagocytosed bacteria was determined by colony counts following treatment with polymyxin B to kill extracellular bacteria. Only 1 to 2% of the phagocytosed bacteria remained viable. Opsonization with heat-inactivated immune serum reduced the amount of attachment and phagocytosis of the bacteria but did not alter survival rates. In contrast to previous reports, these data suggest that phagocytosed B, pertussis bacteria are killed by human neutrophils.
引用
收藏
页码:956 / 959
页数:4
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