Gene silencing using micro-RNA designed hairpins

被引：226

作者：

McManus, MT ^{[1
]}

Petersen, CP ^{[1
]}

Haines, BB ^{[1
]}

Chen, JZ ^{[1
]}

Sharp, PA ^{[1
]}

机构：

[1] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

来源：

RNA | 2002年 / 8卷 / 06期

关键词：

dsRNA; H1; promoter; miRNA; RNAi; siRNA; T-cell;

D O I：

10.1017/S1355838202024032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During RNA interference (RNAi), long dsRNA is processed to similar to21 nt duplexes, short interfering RNAs (siRNAs), which silence genes through a mRNA degradation pathway. Small temporal RNAs (stRNAs) and micro-RNAs (miRNAs) are similar to21 nt RNAs that are processed from endogenously encoded hairpin-structured precursors, and function to silence genes via translational repression. Here we report that synthetic hairpin RNAs that mimic siRNAs and miRNA precursor molecules can target a gene for silencing, and the mechanism of silencing appears to be through mRNA degradation and not translational repression. The sequence and structural configuration of these RNAs are important, and even slight modification In structure can affect the silencing activity of the hairpins. Furthermore, these RNAs are active when expressed by DNA vectors containing polymerase III promoters, opening the possibility for new approaches in stable RNAi-based loss of function studies.

引用

页码：842 / 850

页数：9

共 39 条

[11] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].