A Role for RNAi in the Selective Correction of DNA Methylation Defects

被引：282

作者：

Teixeira, Felipe Karam ^{[1
,2
]}

Heredia, Fabiana ^{[1
]}

Sarazin, Alexis ^{[2
]}

Roudier, Francois ^{[1
,2
]}

Boccara, Martine ^{[1
,2
]}

Ciaudo, Constance ^{[3
,4
]}

Cruaud, Corinne ^{[5
]}

Poulain, Julie ^{[5
]}

Berdasco, Maria ^{[6
]}

Fraga, Mario F. ^{[6
]}

Voinnet, Olivier ^{[3
]}

Wincker, Patrick ^{[5
]}

Esteller, Manel ^{[6
]}

Colot, Vincent ^{[1
,2
]}

机构：

[1] Univ Evry Val Essonne, Inst Natl Rech Agron UMR 1165, CNRS, UMR 8114,Unite Rech Genom Vegetale, F-91057 Evry, France

[2] Ecole Normale Super, CNRS, Dept Biol, UMR 8186, F-75230 Paris 05, France

[3] CNRS, Inst Biol Mol Plantes, Unite Propre Rech 2357, F-67084 Strasbourg, France

[4] Inst Curie, CNRS, UMR 218, F-75248 Paris 05, France

[5] CEA, Inst Genom, F-91057 Evry, France

[6] Ctr Nacl Invest Oncol, Lab Epigenet Canc, Madrid 28029, Spain

来源：

SCIENCE | 2009年 / 323卷 / 5921期

关键词：

EPIGENETIC INHERITANCE; ARABIDOPSIS; EPIGENOME; IV;

D O I：

10.1126/science.1165313

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, which suggests that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis (as in cancer) or meiosis, leading to epiallelic variation. We demonstrated the existence of an efficient mechanism that protects against transgenerational loss of DNA methylation in Arabidopsis. Remethylation is specific to the subset of heavily methylated repeats that are targeted by the RNA interference (RNAi) machinery. This process does not spread into flanking regions, is usually progressive over several generations, and faithfully restores wild-type methylation over target sequences in an RNAi-dependent manner. Our findings suggest an important role for RNAi in protecting genomes against long-term epigenetic defects.

引用

页码：1600 / 1604

页数：5

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