Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation

被引:42
作者
Arantes-Costa, Fernanda M. [1 ]
Lopes, Fernanda D. T. Q. S. [1 ]
Toledo, Alessandra C. [1 ]
Magliarelli-Filho, Pedro A. [1 ]
Moriya, Henrique T. [3 ]
Carvalho-Oliveira, Regiane [2 ]
Mauad, Thais [2 ]
Saldiva, Paulo H. N. [2 ]
Martins, Milton A. [1 ]
机构
[1] Univ Sao Paulo, Sch Med, Dept Med, BR-01246903 Sao Paulo, Brazil
[2] Univ Sao Paulo, Sch Med, Dept Pathol, BR-01246903 Sao Paulo, Brazil
[3] Univ Sao Paulo, Escola Politecn, BR-01246903 Sao Paulo, Brazil
关键词
asthma; experimental asthma models; ROFA; particulate matter; epithelial remodeling; eosinophils; pulmonary responsiveness;
D O I
10.1177/0192623308317427
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
transition metals, which are involved in the pathological effects of PM. The objective of this study was to investigate the effects of intranasal administration of ROFA on pulmonary inflammation, pulmonary responsiveness, and excess mucus production in a mouse model of chronic pulmonary allergic inflammation. BALB/c mice received intraperitoneal injections of ovalbumin (OVA) solution (days 1 and 14). OVA challenges were performed on days 22, 24, 26, and 28. After the challenge, mice were intranasally instilled with ROFA. After forty-eight hours, pulmonary responsiveness was performed. Mice were sacrificed, and lungs were removed for morphometric analysis. OVA-exposed mice presented eosinophilia in the bronchovascular space (p < .001), increased pulmonary responsiveness (p < .001), and epithelial remodeling (p = .003). ROFA instillation increased pulmonary responsiveness (p = .004) and decreased the area of ciliated cells in the airway epithelium (p = .006). The combined ROFA instillation and OVA exposure induced a further increase in values of pulmonary responsiveness (p = .043) and a decrease in the number of ciliated cells in the airway epithelium (p = .017). PM exposure results in pulmonary effects that are more intense in mice with chronic allergic pulmonary inflammation.
引用
收藏
页码:680 / 686
页数:7
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