Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation

被引:42
作者
Arantes-Costa, Fernanda M. [1 ]
Lopes, Fernanda D. T. Q. S. [1 ]
Toledo, Alessandra C. [1 ]
Magliarelli-Filho, Pedro A. [1 ]
Moriya, Henrique T. [3 ]
Carvalho-Oliveira, Regiane [2 ]
Mauad, Thais [2 ]
Saldiva, Paulo H. N. [2 ]
Martins, Milton A. [1 ]
机构
[1] Univ Sao Paulo, Sch Med, Dept Med, BR-01246903 Sao Paulo, Brazil
[2] Univ Sao Paulo, Sch Med, Dept Pathol, BR-01246903 Sao Paulo, Brazil
[3] Univ Sao Paulo, Escola Politecn, BR-01246903 Sao Paulo, Brazil
关键词
asthma; experimental asthma models; ROFA; particulate matter; epithelial remodeling; eosinophils; pulmonary responsiveness;
D O I
10.1177/0192623308317427
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
transition metals, which are involved in the pathological effects of PM. The objective of this study was to investigate the effects of intranasal administration of ROFA on pulmonary inflammation, pulmonary responsiveness, and excess mucus production in a mouse model of chronic pulmonary allergic inflammation. BALB/c mice received intraperitoneal injections of ovalbumin (OVA) solution (days 1 and 14). OVA challenges were performed on days 22, 24, 26, and 28. After the challenge, mice were intranasally instilled with ROFA. After forty-eight hours, pulmonary responsiveness was performed. Mice were sacrificed, and lungs were removed for morphometric analysis. OVA-exposed mice presented eosinophilia in the bronchovascular space (p < .001), increased pulmonary responsiveness (p < .001), and epithelial remodeling (p = .003). ROFA instillation increased pulmonary responsiveness (p = .004) and decreased the area of ciliated cells in the airway epithelium (p = .006). The combined ROFA instillation and OVA exposure induced a further increase in values of pulmonary responsiveness (p = .043) and a decrease in the number of ciliated cells in the airway epithelium (p = .017). PM exposure results in pulmonary effects that are more intense in mice with chronic allergic pulmonary inflammation.
引用
收藏
页码:680 / 686
页数:7
相关论文
共 49 条
[41]   Effects of ambient air pollution on symptom severity and medication use in children with asthma [J].
Slaughter, JC ;
Lumley, T ;
Sheppard, L ;
Koenig, JQ ;
Shapiro, GG .
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2003, 91 (04) :346-353
[42]   Polycyclic aromatic hydrocarbons induce both apoptotic and anti-apoptotic signals in Hepa1c1c7 cells [J].
Solhaug, A ;
Refsnes, M ;
Låg, M ;
Schwarze, PE ;
Husoy, T ;
Holme, JA .
CARCINOGENESIS, 2004, 25 (05) :809-819
[43]   Optimization of route of administration for coexposure to ovalbumin and particle matter to induce adjuvant activity in respiratory allergy in the mouse [J].
Steerenberg, PA ;
van Dalen, WJ ;
Withagen, CET ;
Dormans, JAMA ;
van Loveren, H .
INHALATION TOXICOLOGY, 2003, 15 (13) :1309-1325
[44]   Strain dependence of airway hyperresponsiveness reflects differences in eosinophil localization in the lung [J].
Takeda, K ;
Haczku, A ;
Lee, JJ ;
Irvin, CG ;
Gelfand, EW .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2001, 281 (02) :L394-L402
[45]   The effect of allergen-induced airway inflammation on airway remodeling in a murine model of allergic asthma [J].
Tanaka, H ;
Masuda, T ;
Tokuoka, S ;
Komai, M ;
Nagao, K ;
Takahashi, Y ;
Nagai, H .
INFLAMMATION RESEARCH, 2001, 50 (12) :616-624
[46]   Allergen exposure of mouse airways evokes remodeling of both bronchi and large pulmonary vessels [J].
Törmänen, KR ;
Uller, L ;
Persson, CGA ;
Erjefält, JS .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2005, 171 (01) :19-25
[47]   Cytokines involved in the systemic inflammatory response induced by exposure to particulate Matter air pollutants (PM10) [J].
van Eeden, SF ;
Tan, WC ;
Suwa, T ;
Mukae, H ;
Terashima, T ;
Fujii, T ;
Qui, DW ;
Vincent, R ;
Hogg, JC .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (05) :826-830
[48]   Allergen-induced airway disease is mouse strain dependent [J].
Whitehead, GS ;
Walker, JKL ;
Berman, KG ;
Foster, WM ;
Schwartz, DA .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2003, 285 (01) :L32-L42
[49]   Effects of ambient air pollution on symptoms of asthma in Seattle-area children enrolled in the CAMP study [J].
Yu, OC ;
Sheppard, L ;
Lumley, T ;
Koenig, JQ ;
Shapiro, GG .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2000, 108 (12) :1209-1214