Mouse CD24 as a signaling molecule for integrin-mediated cell binding: Functional and physical association with src-kinases

被引:53
作者
Sammar, M [1 ]
Gulbins, E [1 ]
Hilbert, K [1 ]
Lang, FR [1 ]
Altevogt, P [1 ]
机构
[1] UNIV TUBINGEN,INST PHYSIOL,D-72076 TUBINGEN,GERMANY
关键词
D O I
10.1006/bbrc.1997.6639
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CD24 is a differentiation antigen expressed by murine hematopoietic and neural cells which is linked to the membrane via a glycosylphosphatidylinositol (GPI) anchor. In monocytic ESb-MP cells the molecule serves as a ligand for P-selectin and triggering with CD24 specific antibodies can activate VLA-5/L1-mediated cell adhesion in these cells. We report here that the aggregation is specific for CD24 and not seen with antibodies to the GPI-anchored molecule Thy-1. The Tyr-kinase inhibitor herbimycin can block the aggregation. We studied CD24 associated molecules that might be involved in signal transduction. Antibody-mediated crosslinking of CD24 induced a rapid Tyr-phosphorylation of several cellular proteins in ESb-MP cells which correlated with an elevated activity of p56lck but not p60fyn or MAP-1 kinase. Several phosphorylated proteins were co-immunoprecipitated with CD24. Re-immunoprecipitation allowed the detection of p56lck, p56hck, and p54fyn but not p60fyn, PI-3k, or PLC gamma as a compenent of the CD24 detergent resistant complex. It is suggested that the CD24-associated kinases are involved in the activation of cell aggregation. (C) 1997 Academic Press.
引用
收藏
页码:330 / 334
页数:5
相关论文
共 31 条
[21]  
PETTY R, 1996, IMMUNOL TODAY, V17, P209
[22]   PHOSPHATIDYLINOSITOL MEMBRANE ANCHORS AND T-CELL ACTIVATION [J].
ROBINSON, PJ .
IMMUNOLOGY TODAY, 1991, 12 (01) :35-41
[23]   SIGNALS DETERMINING PROTEIN-TYROSINE KINASE AND GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED PROTEIN TARGETING TO A GLYCOLIPID-ENRICHED MEMBRANE-FRACTION [J].
RODGERS, W ;
CRISE, B ;
ROSE, JK .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (08) :5384-5391
[24]   THE MURINE HEAT-STABLE ANTIGEN - A DIFFERENTIATION ANTIGEN EXPRESSED IN BOTH THE HEMATOLYMPHOID AND NEURAL CELL LINEAGES [J].
ROUGON, G ;
ALTERMAN, LA ;
DENNIS, K ;
GUO, XJ ;
KINNON, C .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (06) :1397-1402
[25]   The L1 adhesion molecule is a cellular ligand for VLA-5 [J].
Ruppert, M ;
Aigner, S ;
Hubbe, M ;
Yagita, H ;
Altevogt, P .
JOURNAL OF CELL BIOLOGY, 1995, 131 (06) :1881-1891
[26]   HEAT-STABLE ANTIGEN (CD24) AS LIGAND FOR MOUSE P-SELECTIN [J].
SAMMAR, M ;
AIGNER, S ;
HUBBE, M ;
SCHIRRMACHER, V ;
SCHACHNER, M ;
VESTWEBER, D ;
ALTEVOGT, P .
INTERNATIONAL IMMUNOLOGY, 1994, 6 (07) :1027-1036
[27]  
SAMMAR M, 1994, BIOCHIM BIOPHYS ACTA, V1337, P287
[28]   PALMITYLATION OF AN AMINO-TERMINAL CYSTEINE MOTIF OF PROTEIN-TYROSINE KINASES P56LCK AND P59FYN MEDIATES INTERACTION WITH GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED PROTEINS [J].
SHENOYSCARIA, AM ;
GAUEN, LKT ;
KWONG, J ;
SHAW, AS ;
LUBLIN, DM .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (10) :6385-6392
[29]   GPI-ANCHORED CELL-SURFACE MOLECULES COMPLEXED TO PROTEIN TYROSINE KINASES [J].
STEFANOVA, I ;
HOREJSI, V ;
ANSOTEGUI, IJ ;
KNAPP, W ;
STOCKINGER, H .
SCIENCE, 1991, 254 (5034) :1016-1019
[30]   THE GENES FOR A MOUSE HEMATOPOIETIC DIFFERENTIATION MARKER CALLED THE HEAT-STABLE ANTIGEN [J].
WENGER, RH ;
AYANE, M ;
BOSE, R ;
KOHLER, G ;
NIELSEN, PJ .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (04) :1039-1046