Simple separation of chicken gonadal primordial germ cells with and without foreign genes

被引：7

作者：

Jeong, DK ^{[1
]}

Hong, YH ^{[1
]}

Han, JY ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Anim Sci & Technol, Suwon 441744, South Korea

来源：

CELL BIOLOGY INTERNATIONAL | 2002年 / 26卷 / 07期

关键词：

primordial germ cell; Ficoll; neo gene; GFP;

D O I：

10.1006/cbir.2002.0928

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Simplicity is the key element of an inexpensive technique described that is superior in performance to previous methods. It can make it the rapid method of choice to obtain reasonable yields of purified primordial germ cells (PGCs) for immediate production of germline chimeric chickens with integrated foreign genes. After Ficoll centrifugation, the purity of PGCs from gonads was 80.9 +/- 0.08% (mechanical) compared with 86.1 +/- 0.19% (enzymatic). GFP gene and lacZ-transduced chicken gonadal primordial germ cells (gPGCs) examined 72 h after transduction had a transfection efficiency of similar to61% and similar to64%, respectively. After 10 days of G418 selection, approximately 90 and 92% of pure gPGCs did not contain other cells following this Ficoll gradient centrifugation method of preparation. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：647 / 651

页数：5

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