Irreversible lysozyme inactivation and aggregation induced by stirring:: kinetic study and aggregates characterisation

被引：19

作者：

Colombié, S

Gaunand, A

Rinaudo, M

Lindet, B

机构：

[1] Ecole Natl Super Mines, CEREP, F-75006 Paris, France

[2] Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 04期

关键词：

enzyme inactivation; lysozyme; lysozyme aggregation; stirring;

D O I：

10.1023/A:1005655005780

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Stirring strongly enhanced irreversible inactivation and aggregation of lysozyme being studied as a model enzyme. From 0 to 740 rpm (equivalent to impeller tip speeds from 0 to 0.77 m s(-1)), the inactivation kinetic constant was proportional to the power imparted by the impeller. Collisions between inactive and native molecules induced inactivation of the latter and led to lysozyme aggregation. These fractal aggregates of lysozyme were made of monomers, dimers and trimers.

引用

页码：277 / 283

页数：7

共 15 条

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