Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platform

被引:94
作者
Cheng, Wei
Klauke, Norbert
Sedgwick, Helen
Smith, Godfrey L.
Cooper, Jonathan M. [1 ]
机构
[1] Univ Glasgow, Dept Elect & Elect Engn, Bioelect Res Ctr, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1039/b608202e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A device based on five individually addressable microelectrodes, fully integrated within a microfluidic system, has been fabricated to enable the real- time measurement of ionic and metabolic fluxes from electrically active, beating single heart cells. The electrode array comprised one pair of pacing microelectrodes, used for field- stimulation of the cell, and three other microelectrodes, configured as an electrochemical lactate microbiosensor, that were used to measure the amounts of lactate produced by the heart cell. The device also allowed simultaneous in-situ microscopy, enabling optical measurements of cell contractility and fluorescence measurements of extracellular pH and cellular Ca2+. Initial experiments aimed to create a metabolic profile of the beating heart cell, and results show well defined excitation- contraction (EC) coupling at different rates. Ca2+ transients and extracellular pH measurements were obtained from continually paced single myocytes, both as a function of the rate of cell contraction. Finally, the relative amounts of intra- and extra- cellular lactate produced during field stimulation were determined, using cell electroporation where necssary.
引用
收藏
页码:1424 / 1431
页数:8
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