Activation of rabbit blood platelets by anandamide through its cleavage into arachidonic acid

被引：33

作者：

Braud, S

Bon, C

Touqui, L

Mounier, C

机构：

[1] Inst Pasteur, Unite Venis, F-75724 Paris 15, France

[2] Inst Pasteur, INSERM U485, Unite Pharmacol Cellulaire, Paris, France

[3] Univ Cergy Pontoise, Dept Biol, Cergy Pontoise, France

来源：

FEBS LETTERS | 2000年 / 471卷 / 01期

关键词：

cannabinoid ligand; platelet activation; amidase activity;

D O I：

10.1016/S0014-5793(00)01359-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anandamide (ANA), a cannabinoid receptor ligand, stimulated platelet aggregation at concentrations similar to those of arachidonic acrid (AA). The aggregating effect of ANA was inhibited by aspirin but not by SR-141716, a cannabinoid receptor antagonist. In addition, HU-210, a cannabinoid receptor agonist, failed to induce platelet activation. Radiolabelling. experiments showed that exogenous ANA was cleaved by platelets into AA through a phenylmethylsulfonyl fluoride (PMSF)-sensitive pathway. In agreement, PMSF was shown to abolish the aggregating effect of ANA, In conclusion, ANA is able to induce platelet activation via its cleavage by a PMSF-sensitive amidase activity, leading to the release of AA which in turn activates platelets. (C) 2000 Federation of European Biochemical Societies.

引用

页码：12 / 16

页数：5

共 42 条

[1] Anandamide - A new look on fatty acid ethanolamides [J].