Marker saturation of the region flanking the gene NSV conferring resistance to the melon necrotic spot Carmovirus (MNSV) in melon

被引：14

作者：

Morales, M

Luís-Arteaga, M

Alvarez, JM

Dolcet-Sanjuan, R

Monfort, A

Arús, P

Garcia-Mas, J

机构：

[1] IRTA, Dept Genet Vegetal, Barcelona 08348, Spain

[2] SIA, Zaragoza 50080, Spain

[3] CSIC, Cid, Inst Biol Mol Barcelona, ES-08034 Barcelona, Spain

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE | 2002年 / 127卷 / 04期

关键词：

Cucumis melo; bulked segregant analysis; disease resistance;

D O I：

10.21273/JASHS.127.4.540

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The recessive allele (nsv) of the NSV gene confers resistance to the Carmovirus melon necrotic spot virus (MNSV) in melon (Cucumis melo L.). Using an F-2 population obtained from the cross between the resistant Korean accession PI161375 and a susceptible line of 'Piel de Sapo', we have mapped the NSV locus to linkage group 11 (G11) of the melon genome. Additional markers closely linked to NSV were developed by bulked segregant analysis (BSA) using a doubled haploid progeny population derived from the same cross. A detailed map of the NSV region was constructed containing 10 markers spanning a distance of 17.7 cM. The nearest flanking markers to NSV were two amplified fragment length polymorphisms (AFLPs) (CTA/ACG-115 and CTA/ACG-120) and one random amplified polymorphic DNA (RAPD) (OPD08-0.80) separated by 5.9 cM. Two more markers, ACC/ACC-110 and OPX15-1.06, cosegregated with NSV.

引用

页码：540 / 544

页数：5

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