A Love-wave biosensor using nucleic acids as ligands

被引：132

作者：

Schlensog, MD

Gronewold, TMA

Tewes, M

Famulok, M

Quandt, E

机构：

[1] Ctr Adv European Studies & Res Caesar, D-53175 Bonn, Germany

[2] Univ Bonn, Kekule Inst Organ Chem & Biochem, D-53121 Bonn, Germany

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2004年 / 101卷 / 03期

关键词：

Love-wave biosensor array; aptamer; thrombin; Rev;

D O I：

10.1016/j.snb.2004.03.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A Love-wave biosensor array has been designed by coupling aptamers to the surface of a Love-wave sensorchip. The sensor chip consists of five single sensor elements and allows label-free, real-time, and quantitative measurements of protein and nucleic acid binding events in concentration-dependent fashion. The biosensor was calibrated for human alpha-thrombin and HIV-1 Rev peptide by binding fluorescently labelled molecules and correlating the mass of the bound molecules to fluorescence intensity. Detection limits of approximately 75 pg/cm(2) were obtained, and analyte recognition was specific. The sensor can easily be regenerated by simple washing steps. We further demonstrate the versatile applicability of the sensor by immobilizing single-stranded DNA (ssDNA) for the detection of the corresponding counter-strand. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：308 / 315

页数：8

共 13 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].