Determination of celecoxib in human plasma by normal-phase high-performance liquid chromatography with column switching and ultraviolet absorbance detection

被引：62

作者：

Rose, MJ ^{[1
]}

Woolf, EJ ^{[1
]}

Matuszewski, BK ^{[1
]}

机构：

[1] Merck Res Labs, Dept Drug Metab, W Point, PA 19486 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 738卷 / 02期

关键词：

celecoxib;

D O I：

10.1016/S0378-4347(99)00552-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the determination of celecoxib in human plasma. Samples were extracted using 3M Empore membrane extraction cartridges and separated under normal-phase HPLC conditions using a Nucleosil-NO2 (150x4.6 mm, 5 mu m) column. Detection was accomplished using UV absorbance at 260 nm. The HPLC method included a column switching procedure, in which late eluting compounds were diverted to waste, to reduce run-time to 12 min. The assay was linear in the concentration range of 25-2000 ng/ml when 1-ml aliquots of plasma were extracted. Recoveries of celecoxib were greater than 91% over the calibration curve range. Intraday precision and accuracy for this assay were 5.7% C.V. or better and within 2.3% of nominal, respectively. The assay was used to analyze samples collected during human clinical studies. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：377 / 385

页数：9

共 18 条

[11] Systemic biosynthesis of prostacyclin by cyclooxygenase (COX)-2: The human pharmacology of a selective inhibitor of COX-2 [J].