Phenylethynylpyrene-labeled oligonucleotide probes for excimer fluorescence SNP analysis of 23S rRNA gene in clarithromycin-resistant Helicobacter pylori strains

被引：14

作者：

Prokhorenko, Igor A.

Malakhov, Andrei D.

Kozlova, Anna A.

Momynaliev, Kuvat

Govorun, Vadim M.

Korshun, Vladimir A.

机构：

[1] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Res Inst Physicochem Med, Moscow 119992, Russia

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2006年 / 599卷 / 1-2期

基金：

俄罗斯基础研究基金会;

关键词：

phenylethynylpyrene; DNA probes; excimer fluorescence; SNP detection; Helicobacter pylori;

D O I：

10.1016/j.mrfmmm.2006.02.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of phenylethynylpyrene excimer forming pair in the design of specific fluorescent probes for determination of A2144G (A2143G and/or A2143C) mutations in 23S rRNA gene of Helicobacter pylori is described. Analysis of fluorescence spectra of model duplexes revealed optimal positions of fluorophore residues in the probe sequences for maximum efficiency of SNP detection. Application of excimer forming probes for analysis of DNA samples isolated from natural bacterial strains of H. pylori was demonstrated. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：144 / 151

页数：8

共 34 条

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