Polyphenolic compounds from Korean Lonicera japonica Thunb. induces apoptosis via AKT and caspase cascade activation in A549 cells

被引：31

作者：

Park, Kwang Il ^{[1
]}

Park, Hyeonsoo ^{[2
]}

Nagappan, Arulkumar ^{[3
,4
]}

Hong, Gyeong Eun ^{[3
,4
]}

Yumnam, Silvia ^{[3
,4
]}

Lee, Ho Jeong ^{[3
,4
]}

Kim, Eun Hee ^{[5
]}

Lee, Won Sup ^{[6
]}

Shin, Sung Chul ^{[7
]}

Kim, Jin A. ^{[8
]}

Lee, Sang Joon ^{[9
]}

Ma, Jin Yeul

Min, Taesun ^{[10
]}

Heo, Jeong Doo ^{[9
]}

Kim, Gon Sup ^{[3
,4
]}

机构：

[1] KIOM, Korean Med KM Applicat Ctr, Daegu, North Gyeongsan, South Korea

[2] DaeguGyeongbuk Med Innovat Fdn DGMIF, Lab Anim Ctr, Daegu 701310, North Gyeongsan, South Korea

[3] Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 660701, South Gyeongsan, South Korea

[4] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Gyeongsan, South Korea

[5] Int Univ Korea, Dept Nursing Sci, Jinju 660759, South Gyeongsan, South Korea

[6] Gyeongsang Natl Univ Hosp, Gyeongnam Reg Canc Ctr, Gyeongsang Natl Univ, Dept Internal Med,Inst Hlth Sci,Sch Med, Jinju 660702, South Gyeongsan, South Korea

[7] Gyeongsang Natl Univ, Life Sci Res Inst, Dept Chem, Jinju 660701, South Gyeongsan, South Korea

[8] Int Univ Korea, Dept Phys Therapy, Jinju 660701, South Gyeongsan, South Korea

[9] Korea Inst Toxicol, Chem Toxic Screening Res Ctr, Gyeongnam Dept Environm Toxicol, Jinju 52834, South Gyeongsan, South Korea

[10] Jeju Natl Univ, Dept Anim Biotechnol, Jeju Do 690756, South Korea

来源：

ONCOLOGY LETTERS | 2017年 / 13卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

anticancer; polyphenolic compounds; Lonicera japonica Thunb; protein kinase B; apoptosis; CYCLE ARREST; LUNG-CANCER; BCL-2; BAX; EPIDEMIOLOGY; EXPRESSION; FLAVONOIDS; INDUCTION; NECROSIS; EXTRACT;

D O I：

10.3892/ol.2017.5771

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Lonicera japonica Thunb. (L. japonica T.) has historically been used in Korean herbal medicine due to its anticancer and protective effects on the respiratory system. In the present study, the polyphenolic compounds in L. japonica T. were investigated using high-performance liquid chromatography coupled with tandem mass spectrometry, and its anticancer effects on A549 non-small-cell lung cancer cells were studied. Polyphenolic compounds potentially inhibit A549 cells in a dose-dependent manner. Flow cytometry and western blot analysis demonstrated that polyphenolic compounds induce apoptosis by regulating the protein expression levels of caspases, poly-(ADP-ribose) polymerase and the B-cell lymphoma-2-associated X-protein/B-cell lymphoma-extra large ratio. Furthermore, polyphenolic compounds inhibited mitochondrial membrane potential activity. Caspase-3 activity was increased in a dose-dependent manner and polyphenolic compounds inhibited the activation of protein kinase B by dephosphorylation. These results suggest that polyphenolic compounds in A549 cells indicate the anticancer activity through the induction of apoptosis.

引用

页码：2521 / 2530

页数：10

共 40 条

[1]

The Role of Phenolic Compounds in the Fight against Cancer - A Review [J].

Carocho, Mrcio ;

Ferreira, Isabel C. F. R. .

ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 2013, 13 (08) :1236-1258

[2]

Antioxidant activities of extracts from five anti-viral medicinal plants [J].

Chen, KL ;

Plumb, GW ;

Bennett, RN ;

Bao, YP .

JOURNAL OF ETHNOPHARMACOLOGY, 2005, 96 (1-2) :201-205

[3]

Choi Eun Jeong, 2008, Nutr Res Pract, V2, P322, DOI 10.4162/nrp.2008.2.4.322

[4]

Mutations of multiple genes cause deregulation of NF-κB in diffuse large B-cell lymphoma [J].

Compagno, Mara ;

Lim, Wei Keat ;

Grunn, Adina ;

Nandula, Subhadra V. ;

Brahmachary, Manisha ;

Shen, Qiong ;

Bertoni, Francesco ;

Ponzoni, Maurilio ;

Scandurra, Marta ;

Califano, Andrea ;

Bhagat, Govind ;

Chadburn, Amy ;

Dalla-Favera, Riccardo ;

Pasqualucci, Laura .

NATURE, 2009, 459 (7247) :717-U124

[5]

Apoptosis: A review of programmed cell death [J].

Elmore, Susan .

TOXICOLOGIC PATHOLOGY, 2007, 35 (04) :495-516

[6]

Expression and regulation of Bcl-2, Bcl-xl, and Bax correlate with p53 status and sensitivity to apoptosis in childhood acute lymphoblastic leukemia [J].

Findley, HW ;

Gu, LB ;

Yeager, AM ;

Zhou, MX .

BLOOD, 1997, 89 (08) :2986-2993

[7]

Lung cancer - 9: Molecular biology of lung cancer: clinical implications [J].

Fong, KM ;

Sekido, Y ;

Gazdar, AF ;

Minna, JD .

THORAX, 2003, 58 (10) :892-900

[8]

Ha GH, 2002, ELECTROPHORESIS, V23, P2513, DOI 10.1002/1522-2683(200208)23:15<2513::AID-ELPS2513>3.0.CO

[9]

2-W

[10]

High concentrations of ascorbic acid induces apoptosis of human gastric cancer cell by p38-MAP kinase-dependent up-regulation of transferrin receptor [J].

Ha, Yu Mi ;

Park, Min Kyu ;

Kim, Hye Jung ;

Seo, Han Geuk ;

Lee, Jae Heun ;

Chang, Ki Churl .

CANCER LETTERS, 2009, 277 (01) :48-54

← 1 2 3 4 →