In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues

被引：42

作者：

Biris, Alexandru S. ^{[1
]}

Galanzha, Ekaterina I. ^{[2
]}

Li, Zhongrui ^{[1
]}

Mahmood, Meena ^{[1
]}

Xu, Yang ^{[1
]}

Zharov, Vladimir P. ^{[2
]}

机构：

[1] Univ Arkansas, Dept Appl Sci, Nanotechnol Ctr, Little Rock, AR 72204 USA

[2] Univ Arkansas Med Sci, Philips Class Laser & Nanomed Labs, Little Rock, AR 72205 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 02期

关键词：

In vivo flow cytometry; Raman spectroscopy; carbon nanotubes; cancer cells; blood and lymph flow; BIOCOMPATIBILITY; BIODISTRIBUTION; NANODIAGNOSTICS; TRANSPORTERS; SPECTROSCOPY; CIRCULATION; DELIVERY; AGENTS;

D O I：

10.1117/1.3119145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanoparticles are intensively being explored as contrast agents for medical diagnostics and therapies using various optical methods. We present the first demonstration of the use of time-resolved Raman spectroscopy for in vivo real-time detection of circulating carbon nanotubes (CNTs) or cancer cells labeled with CNTs in the lymph, blood, and tissues of live animals with fast spectral acquisition times of down to few milliseconds. After intravenously administering CNTs in the tail vein of the rat, this technique provides the ability to detect the circulation of CNTs in the blood microvessels of the intact rat ear. The capability of Raman spectroscopy is also demonstrated to monitor, identify, and image the CNTs during their transportation by lymphatics in the rat ear and mesentery. The strong and specific Raman scattering properties of CNTs make it possible to detect in vitro and in vivo single cancer cells (HeLa) tagged with CNTs. In vivo Raman flow cytometry opens a new avenue for multiparameter analysis of circulating nanoparticles with strong Raman scattering properties and their pharmokinetics in blood and lymph systems. Moreover, this technology has the potential for molecular detection and identification of circulating tumor cells, and infections labeled with CNTs. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3119145]

引用

页数：10

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