Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation

被引:249
作者
Li, S
Harrison, D
Carbonetto, S
Fässler, R
Smyth, N
Edgar, D
Yurchenco, PD
机构
[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pathol & Lab Med, Piscataway, NJ 08854 USA
[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ, Canada
[3] Montreal Gen Hosp, Inst Res, Ctr Res Neurosci, Montreal, PQ H3G 1A4, Canada
[4] Max Planck Inst Biochem, D-8285 Martinsried, Germany
[5] Univ Cologne, Fac Med, Inst Biochem 2, D-50924 Cologne, Germany
[6] Univ Liverpool, Dept Human Anat & Cell Biol, Liverpool L69 3G3E, Merseyside, England
关键词
basement membrane; gastrulation; integrin; dystroglycan; apoptosis;
D O I
10.1083/jcb.200203073
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e., the expression of laminin and companion matrix components, their accumulation on the cell surface and assembly into basement membrane between endoderm and inner cell mass, and the ensuing differentiation of epiblast. In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells null for gamma1-laminin, beta1-integrin and alpha/beta-dystroglycan to dissect the contributions of laminin domains and interacting receptors to this process. We found that (a) laminin enables beta1-integrin-null embryoid bodies to assemble basement membrane and achieve epiblast with beta1-integrin enabling expression of the laminin alpha1 subunit; (b) basement membrane assembly and differentiation require laminin polymerization in conjunction with cell anchorage, the latter critically dependent upon a heparin-binding locus within LG module-4; (c) dystroglycan is not uniquely required for basement membrane assembly or initial differentiation; (d) dystroglycan and integrin cooperate to sustain survival of the epiblast and regulate laminin expression; and (e) laminin, acting via beta1-integrin through LG1-3 and requiring polymerization, can regulate dystroglycan expression.
引用
收藏
页码:1279 / 1290
页数:12
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