Genome wide analysis of TNF-inducible genes reveals that antioxidant enzymes are induced by TNF and responsible for elimination of ROS

被引：28

作者：

Sasazuki, T

Okazaki, T

Tada, K

Sakon-Komazawa, S

Katano, M

Tanaka, M

Yagita, H

Okumura, K

Tominaga, N

Hayashizaki, Y

Okazaki, Y

Nakano, H

机构：

[1] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 1138421, Japan

[2] Kyushu Univ, Grad Sch Med Sci, Dept Canc Therapy & Res, Higashi Ku, Fukuoka 8128582, Japan

[3] Kyushu Univ, Grad Sch Med Sci, Dept Surg & Oncol, Higashi Ku, Fukuoka 8128582, Japan

[4] JST, PRESTO, Kawaguchi, Saitama, Japan

[5] RIKEN, Yokohama Inst, Genom Sci Ctr, Lab Genome Explorat Res Grp,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

MOLECULAR IMMUNOLOGY | 2004年 / 41卷 / 05期

基金：

日本学术振兴会;

关键词：

microarray; NF-kappa B; ROS; TNF; TRAF;

D O I：

10.1016/j.molimm.2004.03.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently showed that TNF induces accumulation of reactive oxygen species (ROS) that mediates necrosis in murine embryonic fibroblasts (MEFs) derived from TRAF2- and TRAF5-double deficient (DKO) mice. To elucidate the defects that subsequently cause accumulation of ROS in DKO MEFs, we compared gene expression profiles of wild-type and DKO MEFs before and after TNF stimulation using cDNA microarrays. Interestingly, many antioxidant enzymes are induced by TNF in wild-type MEFs, induction of these genes is impaired in DKO MEFs. Taken that TNF induces accumulation of ROS in DKO, but not wild-type MEFs, upregulation of antioxidant enzyme(s) might play a crucial role in elimination of ROS. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：547 / 551

页数：5

共 17 条

[11] NF-κB inhibits TNF-induced accumulation of ROS that mediate prolonged MAPK activation and necrotic cell death [J].