Development and validation of a liquid chromatography method for analysis of colistin sulphate

被引：47

作者：

Orwa, JA ^{[1
]}

Van Gerven, A ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Farmaceut Chem & Anal Geneesmiddelen, B-3000 Louvain, Belgium

来源：

CHROMATOGRAPHIA | 2000年 / 51卷 / 7-8期

关键词：

column liquid chromatography; colistin; method validation;

D O I：

10.1007/BF02490480

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The peptide antibiotic Colistin has been successfully separated into more than thirty components on a YMC-Pack Pro, C-18 column with a mobile phase of acetonitrile - sodium sulphate (0.7% m/v)- phosphoric acid (6.8% v/v dilution of 85% m/m H3PO4) - water (21.5:50:5:23.5) at 1.0 mt per min and detection at 215 nm. Baseline resolution of the major components, colistin A and B, and many minor components was obtained. Robustness was evaluated by per forming a full-factorial design experiment. The method showed good selectivity, repeatability, linearity and sensitivity.

引用

页码：433 / 436

页数：4

共 17 条

[11] STUDIES ON CHEMICAL STRUCTURE OF COLISTIN .1. FRACTIONATION, MOLECULAR WEIGHT DETERMINATION, AMINO ACID AND FATTY ACID COMPOSITION [J].