Hollow-Fiber Membrane Chamber as a Device for In Situ Environmental Cultivation

被引：88

作者：

Aoi, Yoshiteru ^{[1
]}

Kinoshita, Tomoyuki ^{[2
]}

Hata, Toru ^{[2
]}

Ohta, Hiroaki ^{[3
]}

Obokata, Haruko ^{[3
]}

Tsuneda, Satoshi ^{[3
]}

机构：

[1] Waseda Univ, Waseda Inst Adv Study, Shinjuku Ku, Tokyo 1628480, Japan

[2] Waseda Univ, Dept Chem Engn, Shinjuku Ku, Tokyo 1698555, Japan

[3] Waseda Univ, Dept Life Sci & Med Biosci, Shinjuku Ku, Tokyo 1628480, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 11期

关键词：

UNCULTURED SOIL BACTERIA; MICROBIAL DIVERSITY; ACTIVATED-SLUDGE; RIBOSOMAL-RNA; PURE CULTURE; MICROORGANISMS; GROWTH; CULTURABILITY; INCUBATION; CELLS;

D O I：

10.1128/AEM.02542-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

引用

页码：3826 / 3833

页数：8

共 35 条

[1] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[2] Incubation of environmental samples in a diffusion chamber increases the diversity of recovered isolates [J].

Bollmann, Annette ;

Lewis, Kim ;

Epstein, Slava S. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (20) :6386-6390

[3] Effect of signal compounds and incubation conditions on the culturability of freshwater bacterioplankton [J].

Bruns, A ;

Nübel, U ;

Cypionka, H ;

Overmann, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :1980-1989

[4] Cyclic AMP and acyl homoserine lactones increase the cultivation efficiency of heterotrophic bacteria from the central Baltic Sea [J].

Bruns, A ;

Cypionka, H ;

Overmann, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) :3978-3987

[5] VIABILITY AND ISOLATION OF MARINE-BACTERIA BY DILUTION CULTURE - THEORY, PROCEDURES, AND INITIAL RESULTS [J].

BUTTON, DK ;

SCHUT, F ;

QUANG, P ;

MARTIN, R ;

ROBERTSON, BR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (03) :881-891

[6] Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria [J].

Cho, JC ;

Giovannoni, SJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (01) :432-440

[7] Global Sequencing: A Review of Current Molecular Data and New Methods Available to Assess Microbial Diversity [J].

Christen, Richard .

MICROBES AND ENVIRONMENTS, 2008, 23 (04) :253-268

[8] High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates [J].

Connon, SA ;

Giovannoni, SJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) :3878-3885

[9] Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria [J].

Davis, KER ;

Joseph, SJ ;

Janssen, PH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (02) :826-834

[10] Microcolony cultivation on a soil substrate membrane system selects for previously uncultured soil bacteria [J].

Ferrari, BC ;

Binnerup, SJ ;

Gillings, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8714-8720

← 1 2 3 4 →