A noninterference polypyrrole glucose biosensor

被引:46
作者
Chen, Cheng [1 ]
Jiang, Yan [1 ]
Kan, Jinqing [1 ]
机构
[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Peoples R China
基金
中国国家自然科学基金;
关键词
noninterference; four-electrode cell; glucose biosensor; ascorbic acid; infrared spectroscopy; ultraviolet spectroscopy;
D O I
10.1016/j.bios.2006.01.023
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In order to eliminate the interference of impurities, such as ascorbic acid, a noninterference polypyrrole glucose biosensor was constructed with a four-electrode cell consisting of a polypyrrole film electrode, a polypyrrole-glucose oxidase electrode, a counter electrode and a reference electrode. The pure catalytic current of glucose oxidase (GOD) can be obtained from the difference between response currents of two working electrodes with and without GOD. The effects of potential, pH and temperature on analytical performance of the glucose biosensor were discussed. The optimum pH and apparent activation energy of enzyme-catalyzed reaction are 5.5 and 25 kJ mol(-1), respectively. The response current of the biosensor increases linearly with the increasing glucose concentration from 0.005 to 20.0 mmol dm(-3). The results show the glucose biosensor with under 2% of relative deviation has good ability of anti -interference. The glucose biosensor was also characterized with Fr-IR and UV-vis spectra. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:639 / 643
页数:5
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