A novel encoded particle technology that enables simultaneous interrogation of multiple cell types

被引：29

作者：

Beske, O ^{[1
]}

Guo, JJ ^{[1
]}

Li, JR ^{[1
]}

Bassoni, D ^{[1
]}

Bland, K ^{[1
]}

Marciniak, H ^{[1
]}

Zarowitz, M ^{[1
]}

Temov, V ^{[1
]}

Ravkin, I ^{[1
]}

Goldbard, S ^{[1
]}

机构：

[1] Vitra Biosci Inc, Mountain View, CA 94043 USA

来源：

JOURNAL OF BIOMOLECULAR SCREENING | 2004年 / 9卷 / 03期

关键词：

multiplexing; imaging; cell-based assays; particle technology; cells; microarrays;

D O I：

10.1177/1087057103260088

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors have developed a cellular analysis platform, based on encoded microcarriers, that enables the multiplexed analysis of a diverse range of cellular assays. At the core of this technology are classes of microcarriers that have unique, identifiable codes that are deciphered using CCD-based imaging and subsequent image analysis. The platform is compatible with a wide variety of cellular imaging-based assays, including calcium flux, reporter gene activation, cytotoxicity, and proliferation. In addition, the platform is compatible with both colorimetric and fluorescent readouts. Notably, this technology has the unique ability to multiplex different cell lines in a single microplate well, enabling scientists to perform assays and data analysis in novel ways.

引用

页码：173 / 185

页数：13

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