Characterisation of CTX-M and AmpC genes in human isolates of Escherichia coli identified between 1995 and 2003 in England and Wales

被引:47
作者
Hopkins, K. L.
Batchelor, M. J.
Liebana, E.
Deheer-Graham, A. P.
Threlfalla, E. J.
机构
[1] Hlth Protect Agcy, Ctr Infect, Lab Enter Pathogens, Salmonella Reference Unit, London NW9 5EQ, England
[2] Vet Labs Agcy, Dept Food & Environm Safety, Weybridge KT15 3NB, Surrey, England
关键词
CTX-M; AmpC; beta-lactamases; Escherichia coli; antimicrobial resistance; ampC promoter;
D O I
10.1016/j.ijantimicag.2006.03.027
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
CTX-M and AmpC genes in human isolates of Escherichia coli, their genetic environment and their host plasmids were examined. Isolates (n = 103) were selected based on resistance (minimum inhibitory concentration (MIC) >= 1 mu g/mL) to ceftriaxone and cefotaxime. Polymerase chain reaction (PCR) and sequencing identified 29 isolates containing bla(CTX-M-15), 1 each of bla(CTX-M-2) (a strain originating from Israel) and bla(CTX-M-40), 20 isolates containing bla(CMY-7), 4 bla(CMY-2) and 1 bla(CMY-21). This is the first study of plasmid-mediated AmpC genes in E. coli in the UK. Eleven cefoxitin-resistant, AmpC PCR-negative isolates had ampC promoter region mutations. All bla(CTX-M-15) and 24 of 25 bla(CMY) genes were associated with an ISEcpl-like element. The bla(CTX-M-2) was located in an orf513-bearing class 1 integron. Plasmid restriction digests suggest transfer of genes between different plasmid backbones. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:180 / 192
页数:13
相关论文
共 43 条
[31]   Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR [J].
Pérez-Pérez, FJ ;
Hanson, ND .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2153-2162
[32]   Plasmid-determined AmpC-type β-lactamases [J].
Philippon, A ;
Arlet, G ;
Jacoby, GA .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (01) :1-11
[33]   Insertion sequence ISEcp1B is involved in expression and mobilization of a blaCTX-M β-lactamase gene [J].
Poirel, L ;
Decousser, JW ;
Nordmann, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (09) :2938-2945
[34]   Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum β-lactamase CTX-M-15 and of its structurally related β-lactamase CTX-M-3 [J].
Poirel, L ;
Gniadkowski, M ;
Nordmann, P .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 50 (06) :1031-1034
[35]  
POWELL NG, 1994, FEMS MICROBIOL LETT, V119, P193, DOI 10.1016/0378-1097(94)90413-8
[36]   Description of In116, the first blaCTX-M-2-containing complex class 1 integron found in Morganella morganii isolates from Buenos Aires, Argentina [J].
Power, P ;
Galleni, M ;
Di Conza, J ;
Ayala, JA ;
Gutkind, G .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (04) :461-465
[37]   Novel complex sul1-type integron in Escherichia coli carrying blaCTX-M-9 [J].
Sabaté, M ;
Navarro, F ;
Miró, E ;
Campoy, S ;
Mirelis, B ;
Barbé, J ;
Prats, G .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (08) :2656-2661
[38]   Integron-mediated ESBL resistance in rare serotypes of Escherichia coli causing infections in an elderly population of Israel [J].
Sompolinsky, D ;
Nitzan, Y ;
Tetry, S ;
Wolk, M ;
Vulikh, I ;
Kerrn, MB ;
Sandvang, D ;
Hershkovits, G ;
Katcoff, DJ .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (01) :119-122
[39]   Carbapenem resistance in Escherichia coli associated with plasmid-determined CMY-4 β-lactamase production and loss of an outer membrane protein [J].
Stapleton, PD ;
Shannon, KP ;
French, GL .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (05) :1206-1210
[40]  
Walther-Rasmussen J, 2004, CAN J MICROBIOL, V50, P137, DOI [10.1139/w03-111, 10.1139/W03-111]