CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

被引：77

作者：

Ting, AH

Jair, KW

Suzuki, H

Yen, RWC

Baylin, SB ^{[1
]}

Schuebel, KE

机构：

[1] Johns Hopkins Univ, Program Cellular & Mol Med, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Canc Biol Div, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21231 USA

来源：

NATURE GENETICS | 2004年 / 36卷 / 06期

关键词：

D O I：

10.1038/ng1365

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

引用

页码：582 / 584

页数：3

共 15 条

[1] GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer
Akiyama, Y
Watkins, N
Suzuki, H
Jair, KW
van Engeland, M
Esteller, M
Sakai, H
Ren, CY
Yuasa, Y
Herman, JG
Baylin, SB
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (23) : 8429 - 8439
[2] A system for stable expression of short interfering RNAs in mammalian cells
Brummelkamp, TR
Bernards, R
Agami, R
[J]. SCIENCE, 2002, 296 (5567) : 550 - 553
[3] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS
FROMMER, M
MCDONALD, LE
MILLAR, DS
COLLIS, CM
WATT, F
GRIGG, GW
MOLLOY, PL
PAUL, CL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) : 1827 - 1831
[4] Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands
Herman, JG
Graff, JR
Myohanen, S
Nelkin, BD
Baylin, SB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) : 9821 - 9826
[5] Maintenance of genomic methylation requires a SW12/SNF2-like protein
Jeddeloh, JA
Stokes, TL
Richards, EJ
[J]. NATURE GENETICS, 1999, 22 (01) : 94 - 97
[6] The fundamental role of epigenetic events in cancer
Jones, PA
Baylin, SB
[J]. NATURE REVIEWS GENETICS, 2002, 3 (06) : 415 - 428
[7] TARGETED MUTATION OF THE DNA METHYLTRANSFERASE GENE RESULTS IN EMBRYONIC LETHALITY
LI, E
BESTOR, TH
JAENISCH, R
[J]. CELL, 1992, 69 (06) : 915 - 926
[8] Myöhänen SK, 1998, CANCER RES, V58, P591
[9] Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
Nan, XS
Ng, HH
Johnson, CA
Laherty, CD
Turner, BM
Eisenman, RN
Bird, A
[J]. NATURE, 1998, 393 (6683) : 386 - 389
[10] RNA interference: the new somatic cell genetics?
Paddison, PJ
Hannon, GJ
[J]. CANCER CELL, 2002, 2 (01) : 17 - 23

← 1 2 →