UHPLC-ESI-QTOF-MS-based metabolic profiling of Vicia faba L. (Fabaceae) seeds as a key strategy for characterization in foodomics

被引:86
作者
Abu-Reidah, Ibrahim M. [1 ,2 ,3 ]
del Mar Contreras, Maria [1 ,2 ]
Arraez-Roman, David [1 ,2 ]
Fernandez-Gutierrez, Alberto [1 ,2 ]
Segura-Carretero, Antonio [1 ,2 ]
机构
[1] Univ Granada, Dept Analyt Chem, Fac Sci, E-18071 Granada, Spain
[2] PTS Granada, Funct Food Res & Dev Ctr CIDAF, Granada, Spain
[3] Biodivers & Environm Res Ctr BERC, Til, Nablus, Israel
关键词
Broad bean (Vicia faba L.); Dietary phytochemicals; Foodomics; Phenolic composition; UHPLC-MS/MS; FLIGHT MASS-SPECTROMETRY; HIGH-THROUGHPUT ANALYSIS; LENS-CULINARIS L; LIQUID-CHROMATOGRAPHY; ONOBRYCHIS-VICIIFOLIA; PLANT HORMONES; ANTIOXIDANT CAPACITY; PHENOLIC-COMPOUNDS; IMMATURE SEEDS; MS/MS METHOD;
D O I
10.1002/elps.201300646
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Vicia faba (Fabaceae) is a popular food in many countries and a good source of nutrients. However, little is known about its phytochemical composition, specially referring to phenolic compounds. In the present work, the dietary metabolites from a hydro-methanolic extract of V. faba seeds were thoroughly characterized by a nontargeted analytical approach based on reversed-phase ultra-HPLC (UHPLC) coupled to QTOF-MS. A total of 155 primary and secondary metabolites of various structural types were characterized: carbohydrates, amino acids, organic acids, alkaloids, terpenoids, jasmonates, and, mainly, polyphenols. Among the latter group, 73 compounds were characterized for the first time in this legume. In addition, 24 new structures, belonging to jasmonates and glycosylated N-containing compounds, were also proposed. Thus, this methodology could be implemented in foodomics as a characterization strategy to complement the knowledge of the phytochemical composition of vegetables.
引用
收藏
页码:1571 / 1581
页数:11
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