Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains

被引:109
作者
Sonderegger, M
Jeppsson, M
Larsson, C
Gorwa-Grauslund, MF
Boles, E
Olsson, L
Spencer-Martins, I
Hahn-Hägerdal, B
Sauer, U [1 ]
机构
[1] ETH, Inst Biotechnol, CH-8093 Zurich, Switzerland
[2] Lund Univ, Dept Appl Microbiol, SE-22100 Lund, Sweden
[3] Goethe Univ Frankfurt, Inst Mikrobiol, D-60439 Frankfurt, Germany
[4] Tech Univ Denmark, Bioctr, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark
[5] Univ Nova Lisboa, Fac Sci & Technol, CREM, P-2829516 Caparica, Portugal
关键词
bioprocess; pentose metabolism; ethanol production; lignocellulose; xylose;
D O I
10.1002/bit.20094
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lignocellulose hydrolysate is an abundant substrate for bioethanol production. The ideal microorganism for such a fermentation process should combine rapid and efficient conversion of the available carbon sources to ethanol with high tolerance to ethanol and to inhibitory components in the hydrolysate. A particular biological problem are the pentoses, which are not naturally metabolized by the main industrial ethanol producer Saccharomyces cerevisiae. Several recombinant, mutated, and evolved xylose fermenting S. cerevisiae strains have been developed recently. We compare here the fermentation performance and robustness of eight recombinant strains and two evolved populations on glucose/xylose mixtures in defined and lignocellulose hydrolysate-containing medium. Generally, the polyploid industrial strains depleted xylose faster and were more resistant to the hydrolysate than the laboratory strains. The industrial strains accumulated, however, up to 30% more xylitol and therefore produced less ethanol than the haploid strains. The three most attractive strains were the mutated and selected, extremely rapid xylose consumer TMB3400, the evolved C5 strain with the highest achieved ethanol titer, and the engineered industrial F12 strain with by far the highest robustness to the lignocellulosic hydrolysate. (C) 2004 Wiley Periodicals, Inc.
引用
收藏
页码:90 / 98
页数:9
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