An amperometric biosensor for determining amino acids using a bienzymatic system containing amino acid oxidase and protease

被引:23
作者
Kwan, RCH
Chan, CY
Renneberg, R
机构
[1] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China
[2] Sino German Nanoanalyt Lab, Kowloon, Hong Kong, Peoples R China
关键词
amperometric biosensor; aspartame; L-amino acid oxidase; PCS gel; pronase;
D O I
10.1023/A:1016163315804
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An amperometric biosensor for rapid determination of the concentration of L-amino acids has been developed using L-amino acid oxidase (L-AAO) immobilized by gel entrapment with poly( carbamoyl) sulfonate hydrogel. The broad substrate range of L-AAO allows this biosensor to be flexible in application. The artificial sweetener, aspartame, was determined by coupling L-AAO with pronase.
引用
收藏
页码:1203 / 1207
页数:5
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