An amperometric biosensor for determining amino acids using a bienzymatic system containing amino acid oxidase and protease

被引：23

作者：

Kwan, RCH

Chan, CY

Renneberg, R

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China

[2] Sino German Nanoanalyt Lab, Kowloon, Hong Kong, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 14期

关键词：

amperometric biosensor; aspartame; L-amino acid oxidase; PCS gel; pronase;

D O I：

10.1023/A:1016163315804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An amperometric biosensor for rapid determination of the concentration of L-amino acids has been developed using L-amino acid oxidase (L-AAO) immobilized by gel entrapment with poly( carbamoyl) sulfonate hydrogel. The broad substrate range of L-AAO allows this biosensor to be flexible in application. The artificial sweetener, aspartame, was determined by coupling L-AAO with pronase.

引用

页码：1203 / 1207

页数：5

共 16 条

[11] Determination of underivatized amino acids by high-performance liquid chromatography and electrochemical detection at an amino acid oxidase immobilized CuPtCl6 modified electrode [J].