C2AB: A Molecular Glue for Lipid Vesicles with a Negatively Charged Surface

被引：30

作者：

Diao, Jiajie ^{[1
]}

Yoon, Tae-Young ^{[2
,3
]}

Su, Zengliu ^{[4
]}

Shin, Yeon-Kyun ^{[4
]}

Ha, Taekjip ^{[1
,5
]}

机构：

[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[2] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Inst BioCentury, Taejon 305701, South Korea

[4] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

[5] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

来源：

LANGMUIR | 2009年 / 25卷 / 13期

基金：

美国国家卫生研究院;

关键词：

FUSION; SYNAPTOTAGMIN; LIPOSOMES;

D O I：

10.1021/la901676e

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Artificial particulate systems Such as lipid vesicles are found in a variety of biomedical applications such as drug delivery and targeting. More versatile layers of control would be added if liposomes could be glued together on demand while stabilized against fusion. Here, we present a two-component molecular glue composed of a protein and calcium ions, with each component specialized for fast and specific binding to negatively charged lipid membranes. Upon mixing the two components, the high affinity binding of this glue starts to tightly bridge two lipid vesicles on a subsecond scale. Furthermore, highly charged liposomes are beneficial in preventing spontaneous fusion before applying the molecular glue.

引用

收藏

页码：7177 / 7180

页数：4

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