Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans-Splicing Using Only One Robust DnaE Intein

被引：57

作者：

Busche, Alena E. L. ^{[2
,3
]}

Aranko, A. Sesija ^{[1
]}

Talebzadeh-Farooji, Mehdi ^{[2
,3
]}

Bernhard, Frank ^{[2
,3
]}

Doetsch, Volker ^{[2
,3
]}

Iwai, Hideo ^{[1
]}

机构：

[1] Univ Helsinki, Inst Biotechnol, Res Program Struct Biol & Biophys, Helsinki 00014, Finland

[2] Univ Frankfurt, Inst Biophys Chem, D-60438 Frankfurt, Germany

[3] Univ Frankfurt, Ctr Biomol Magnet Resonance & Cluster Excellence, D-60438 Frankfurt, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 33期

关键词：

inteins; isotopic labeling; NMR spectroscopy; protein modifications; protein splicing; SPLIT INTEIN; IN-VIVO; NOSTOC-PUNCTIFORME; CHEMICAL-SYNTHESIS; NMR-SPECTROSCOPY; LARGER PROTEIN; CURACIN-A; LIGATION; GENE; CRYSTALLIZATION;

D O I：

10.1002/anie.200901488

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A split intein saves nine: A naturally split intein is used to create an efficient three-way ligation method that does not require any refolding steps. This method enables the selective labeling of a central domain within a three-domain protein, with NMR active isotopes (see picture) allowing domain-domain interactions in multidomain proteins to be investigated. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6128 / 6131

页数：4

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