Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia

被引:25
作者
Aboudharam, G [1 ]
Lascola, B [1 ]
Raoult, D [1 ]
Drancourt, M [1 ]
机构
[1] Univ Mediterranee, Fac Med, CNRS, UPRESA 6020,Unite Rickettsies, F-13385 Marseille 05, France
关键词
dental pulp; colonization; infection; bacteria; Coxiella burnetii;
D O I
10.1006/mpat.1999.0343
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Colonization of dental pulp by blood-borne bacteria in the absence of previous inflammation has been hypothetized but has never been convincingly demonstrated. In order to provide convincing support for this hypothesis we attempted to detect Coxiella burnetii DNA in the dental pulp of bacteremic, intraperitoneally inoculated guinea-pigs by PCR amplification and direct sequencing of two molecular targets. Coxiella burnetii DNA was recovered from 20-50% of the animals depending on the molecular target, from 15-20 days after experimental challenge. These results demonstrated, for the first time, that dental pulp is contaminated by blood-borne bacteria and can be detected by molecular tools. (C) 2000 Academic Press.
引用
收藏
页码:249 / 254
页数:6
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