Characterisation of the meningococcal transferrin binding protein complex by photon correlation spectroscopy

被引：21

作者：

Boulton, IC

Gorringe, AR

Carr, RJG

Gorinsky, B

Joannou, CL

Evans, RW

机构：

[1] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP, DIV BIOCHEM & MOL BIOL, LONDON SE1 9RT, ENGLAND

[2] UNIV LONDON BIRKBECK COLL, DEPT CRYSTALLOG, LONDON WC1E 7HX, ENGLAND

[3] PUBL HLTH LAB SERV, CTR APPL MICROBIOL & RES, SALISBURY SP4 0JG, WILTS, ENGLAND

[4] MACRANAL LTD, SALISBURY SP4 6BU, WILTS, ENGLAND

来源：

FEBS LETTERS | 1997年 / 414卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

transferrin; Neisseria meningitidis; photon correlation spectroscopy;

D O I：

10.1016/S0014-5793(97)01025-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species under,vent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar apparent sizes but showed distinctive size profiles suggesting that TbpA forms a largely homogeneous population while TbpB may produce more variable particle sizes under these conditions. (C) 1997 Federation of European Biochemical Societies.

引用

页码：409 / 413

页数：5

共 22 条

[11] PREPARATION AND ANALYSIS OF ISOGENIC MUTANTS IN THE TRANSFERRIN RECEPTOR PROTEIN GENES, TBPA AND TBPB, FROM NEISSERIA-MENINGITIDIS [J].