Correlation between structure and transfection efficiency: a study of DC-Chol-DOPE/DNA complexes

被引：58

作者：

Congiu, A

Pozzi, D

Esposito, C

Castellano, C

Mossa, G

机构：

[1] Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, INFM, I-00185 Rome, Italy

[3] CNR, Ist Neurobiol & Med Mol, I-00133 Rome, Italy

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2004年 / 36卷 / 01期

关键词：

cationic lipids; lipoplexes; X-ray diffraction; transfection efficiency; lipid toxicity;

D O I：

10.1016/j.colsurfb.2004.04.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The supramolecular structural nature of some cationic liposomes-DNA complexes, currently used as vehicles in non-viral gene delivery, has been elucidated by recent X-ray diffraction experiments. The relationship between the chemico-physical properties of these self-assembled structures and their transfection efficiency is extensively studied. Here we report a first comprehensive structural study by using energy dispersive X-ray diffraction, of the complex DC-Chol-DOPE/DNA (3beta[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol dioleoylphosphatidylethanolamine/DNA), which has been classified as one of the most effective in in-vivo experiments. Our results show that DC-Chol-DOPE/DNA lipoplexes have a columnar inverted hexagonal structure, which is not influenced by the cationic liposome/DNA charge ratio. The transfection efficiency of C6 rat glioma cells by DC-Chol-DOPE/DNA lipoplexes and the toxicity of lipoplexes to cells are dramatically affected by cationic liposome/DNA weight ratio. It seems therefore that the lipoplex structures have not any influence on transfection efficiency and toxicity in our experimental system. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：43 / 48

页数：6

共 32 条

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